Rapid and reliable detection methods for food borne pathogen are the key points for food safety control.However

traditional culture based procedure for bacterial identification

generally taking 5 to 7 days and requiring skilled person and non portable equipment

is incompetent for point of care or on site detection.Thus

several isothermal amplification methods characterized by rapidness and thermal cycle independent were developed to fulfill some specific needs for point of care or on site detection.In this review

mechanisms for isothermal amplification methods

designated as loop mediated isothermal amplification

rolling circle amplification

strand displacement amplification

nicking enzyme signal amplification

exonuclease Ⅲ assisted amplification

were explained and compared in detail.Besides

some drawbacks shared by these methods were highlighted

and ways to overcoming those drawbacks such as adoption of propidium monoazide

novol fluorescent dye

high throughput pretreatment equipment and microfluidic chip were provided based on the recent publications of the authors group and others.