A high performance liquid chromatography-mass spectrometric(HPLC-MS) method was developed for the determination of bovine typeⅠ collagen.Collagen sponge samples were digested with trypsin

then the digesting mixture was separated with a C18 column.The detection was performed with electrospray ionization in positive mode and using single ion monitoring(SIM) mode for data collection

and the quantitation was achieved by external standard method.A marker peptide GFSGLDGAK was obtained in the tryptic digestion of bovine typeⅠ collagen.There was a good linearity for the marker peptide in the concentration range of 0.645-21.5 μg/mL(r2=0.999 4).The quantitation limit of the method was 6.45×10-4 μg/mL.Recoveries for the target at three spiked levels of 3.10

2.48 and 1.55 μg/mL ranged from 98.2% to 102%

with relative standard deviations(n=6) of 0.80%.The collagen sponge samples were tested for uniformity

stability and constant value to ensure the effectiveness of the method and the quality of the product

and the content of bovine typeⅠ collagen was determined to be （0.926±0.014） mg/mg.The method is simple

rapid

replicable and high stable

and could meet the requirements for determination of bovine typeⅠ collagen.