A rapid visual method based on aptamer sensor was established for screening of illegally added salbutamol（SAL） in health products.The detection element of this sensor was the SAL-aptamer

and the affinity between SAL-aptamer and ssDNA-binding proteins（SSB） acted as a “switch” of the sensor.SAL-aptamer modified micromagnatic beads（MB）（MB-aptamer）

and horseradish peroxidase（HRP） and SSB jointly modified silica microsphere（SiO2@SSB@HRP） were prepared

respectively.Then MB-aptamer and SiO2@SSB@HRP were connected by the affinity between SAL-aptamer and SSB.SAL

as a key

triggered the “switch” to result in disconnection between SSB and SAL-aptamer during the inspection process

and the free SiO2@SSB@HRP was separated by magnetic action to carry out a color reaction.Under the optimized conditions

the color reaction showed visible gradient variation according to the change of SAL concentration.The color reaction catalyzed by HRP was quantitatively characterized with an ultraviolet-visible spectrophotometer

there was a linear relationship for absorbance wtih SAL concentration in the range of 0.1-10 μmol/L（r2=0.995 9）

with a detection limit of 0.1 μmol/L.The mean recoveries at three spiked levels were all in the range of 86.0%-106%

with relative standard deviations of 3.9%-12%.The method is simple

sensitive and rapid

and it could meet the requirements for the rapid screening of illegally added SAL in real samples.