Chiral covalent organic frameworks(COFs) of CTpPa-1 with two dimensional eclipsed layered sheet structure was synthesized from p phenylenediamine(Pa-1) and chiral organic monomer CTp obtained by the reaction of 1

3

5-triformyloroglucinol(Tp) and (+) -diacetyl-L-tartaric anhydride.The CTpPa-1 was characterized by powder X-ray diffraction(PXRD)

Fourier transform infrared spectroscopy(FT-IR)

scanning electron microscopy(SEM) and nitrogen adsorption.The CTpPa-1 were mixed with C18 to use as a stationary phase for fabricating a packed chiral high performance liquid chromatography(HPLC) column.The resolution ability of the chiral column for different chiral compounds were investigated under different ratios of n hexane-isopropanol as mobile phase.Results showed that the chiral column exhibited a good resolving ability towards 18 racemates

including alcohols

ketones

phenols

organic acids and amines

among which five chiral compounds reached the baseline separation

and seven chiral compounds were close to the baseline separation.The highest resolution(RS) was 3.65.Some positional isomers including nitrophenol

bromoaniline and dinitrobenzene could be separated on the chiral column.Finally

the reproducibility and stability of the chiral column were evaluated.The relative standard deviations(RSD) for retention time and peak area after 50

100

150

200

250 injections of 1-(4-chlorophenyl) ethanol were 0.86% and 1.1%，respectively

indicating that the chiral column had good reproducibility and stability.