Vitamin D is a fat soluble vitamin essential to human health

of which 25 hydroxyvitamin D is the major circulation and storage mode in human body.Dietary intake is an important way to obtain vitamin D and 25-hydroxyvitamin D for human.The pretreatment methods for vitamin D and 25-hydroxyvitamin D in foods usually include alkaline saponification

organic solvent extraction and purification by solid phase extraction or semi preparative chromatography.Traditionally

radioimmunoassay technique and high performance liquid chromatography were used for the determination of vitamin D and 25-hydroxyvitamin D in foods.Nowadays

liquid chromatography tandem mass spectrometry with higher accuracy and precision has become one of the most important methods in determination of vitamin D and 25-hydroxyvitamin D in foods.Recently

two dimensional liquid chromatography and ultrahigh performance supercritical fluid chromatography have gradually showed a powerful potential in detection of vitamin D and 25-hydroxyvitamin D in foods for their great separation abilities.The determination techniques for vitamin D and 25-hydroxyvitamin D and their content distributions in animal and plant foods are summarized in this review

in order to provide a reference for future studies such as developing suitable determination methods for different kinds of foods

determining their contents

providing reasonable diet suggestions for residents and dietary intake assessment.