Analysis of Differential Components of Ganqingqinglan from Different Regions in Tibet Based on UHPLC-Q/Orbitrap HRMS Technology

DUAN Ting-yin; DAWA Drolma; LAN Jun; LI Xue-min; ZHANG Meng-jiao; ZHOU Yan; DENG Fang

doi:10.19969/j.fxcsxb.23062002

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Analysis of Differential Components of Ganqingqinglan from Different Regions in Tibet Based on UHPLC-Q/Orbitrap HRMS Technology

Scientific Papers | 更新时间：2023-10-18

- Analysis of Differential Components of Ganqingqinglan from Different Regions in Tibet Based on UHPLC-Q/Orbitrap HRMS Technology
- Journal of Instrumental Analysis Vol. 42, Issue 10, Pages: 1265-1271(2023)
- 作者机构：
  
  1.成都中医药大学药学院，西南特色中药资源国家重点实验室，四川成都 611137
  2.西藏自治区食品药品检验研究院中药（藏药）质量控制重点实验室，西藏拉萨 850000
  3.中国科学院成都生物研究所，四川成都 610041
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.23062002
  CLC：
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段婷引,达娃卓玛,兰钧等.基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱对西藏不同产地甘青青兰的差异性成分分析[J].分析测试学报,2023,42(10):1265-1271.

DUAN Ting-yin,DAWA Drolma,LAN Jun,et al.Analysis of Differential Components of Ganqingqinglan from Different Regions in Tibet Based on UHPLC-Q/Orbitrap HRMS Technology[J].Journal of Instrumental Analysis,2023,42(10):1265-1271.
段婷引,达娃卓玛,兰钧等.基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱对西藏不同产地甘青青兰的差异性成分分析[J].分析测试学报,2023,42(10):1265-1271. DOI： 10.19969/j.fxcsxb.23062002.

DUAN Ting-yin,DAWA Drolma,LAN Jun,et al.Analysis of Differential Components of Ganqingqinglan from Different Regions in Tibet Based on UHPLC-Q/Orbitrap HRMS Technology[J].Journal of Instrumental Analysis,2023,42(10):1265-1271. DOI： 10.19969/j.fxcsxb.23062002.

摘要

采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱（UHPLC-Q/Orbitrap HRMS）技术对西藏3个地区的41批甘青青兰样本进行分析，通过Compound Discoverer 3.2软件对化学成分进行鉴定，并利用多元统计学分析筛选出不同地区样本之间的差异性成分。在甘青青兰样本中共鉴定73种化合物，包括41种黄酮类、15种有机酸类、8种氨基酸类以及9种其他类化合物。经多元统计学分析，山南市样本（SN）与拉萨市样本（LS）之间有11种差异化合物，山南市样本（SN）与昌都市样本（CD）之间有18种差异化合物，拉萨市样本（LS）与昌都市样本（CD）之间有21种差异化合物（VIP,>,1，,P,<,0.05）。大多数差异化合物为黄酮类及有机酸类化合物。绿原酸在3组样本间的含量差异最大，在CD组含量最高（约为11.85 mg/g），LS组次之（约为7.85 mg/g），SN组最低（约为5.30 mg/g），这与甘青青兰样本的叶子数量和粉末颜色有一定的相关性。结合各地区地势及气候推测光照日长及日温差对甘青青兰化学成分有影响。综上所述，推测地理环境的差异对甘青青兰的性状和化学成分具有影响。研究结果为甘青青兰质量控制及后续开发利用提供了理论基础。

Abstract

This study used ultra-high performance liquid chromatography-quadrupole-orbitrap high-resolution mass spectrometry（UHPLC-Q/Orbitrap HRMS） technology to analyze 41 batches of Ganqingqinglan samples from three regions in Tibet. Compound Discoverer 3.2 software was utilized to identify the chemical components，and differential compounds between different regions were screened through multivariate statistical analysis. A total of 73 compounds were identified in the Ganqingqinglan samples，including 41 flavonoids，15 organic acids，8 amino acids，and 9 other compounds. Through multivariate statistical analysis，11 differential compounds were found between samples from Shannan City（SN） and Lhasa City（LS），18 differential compounds between SN and Qamdo City（CD），and 21 differential compounds between LS and CD（VIP,>,1，,P,<,0.05）. The majority of differential compounds were flavonoids and organic acids. Chlorogenic acid exhibited the largest difference among the three groups，with the highest content found in the CD group（approximately 11.85 mg/g），followed by the LS group（approximately 7.85 mg/g），and the lowest content in the SN group（approximately 5.30 mg/g）. This correlated with the leaf quantity and powder color of the Ganqingqinglan samples. Considering the topography and climate of each region，it is speculated that sunlight duration and diurnal temperature range have an influence on the chemical composition of Ganqingqinglan. In conclusion，geographical environmental differences are believed to have an impact on the characteristics and chemical composition of Ganqingqinglan. These research findings provide a theoretical basis for quality control and subsequent rational development and utilization of Ganqingqinglan.

关键词

UHPLC-Q/Orbitrap HRMS技术甘青青兰多元统计学差异性成分

Keywords

UHPLC-Q/Orbitrap HRMS technologyGanqingqinglanmultivariate statistical analysisdifferential components

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