Simultaneous Determination of 212 Pesticide Residues in <italic style="font-style: italic">Panax Notoginseng</italic> by Gas Chromatography-Tandem Mass Spectrometry

WANG Yi-fan; WU Yi-feng; XU Dun-ming; CHEN Da-jie; ZHENG Wen-jie; JI Chao

doi:10.19969/j.fxcsxb.23033101

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Simultaneous Determination of 212 Pesticide Residues in Panax Notoginseng by Gas Chromatography-Tandem Mass Spectrometry

Scientific Papers | 更新时间：2023-10-18

- Simultaneous Determination of 212 Pesticide Residues in Panax Notoginseng by Gas Chromatography-Tandem Mass Spectrometry
- Journal of Instrumental Analysis Vol. 42, Issue 10, Pages: 1251-1264(2023)
- 作者机构：
  
  1.福建农林大学食品科学学院，福建福州 350002
  2.厦门海关技术中心，福建厦门 361026
  3.天津师范大学生命科学学院，天津 300382
  4.云南农业大学云南生物资源保护与利用国家重点实验室，云南昆明 650091
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.23033101
  CLC：
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王一帆,吴易峰,徐敦明等.气相色谱-串联质谱法同时测定三七中212种农药残留[J].分析测试学报,2023,42(10):1251-1264.

WANG Yi-fan,WU Yi-feng,XU Dun-ming,et al.Simultaneous Determination of 212 Pesticide Residues in Panax Notoginseng by Gas Chromatography-Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2023,42(10):1251-1264.
王一帆,吴易峰,徐敦明等.气相色谱-串联质谱法同时测定三七中212种农药残留[J].分析测试学报,2023,42(10):1251-1264. DOI： 10.19969/j.fxcsxb.23033101.

WANG Yi-fan,WU Yi-feng,XU Dun-ming,et al.Simultaneous Determination of 212 Pesticide Residues in Panax Notoginseng by Gas Chromatography-Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2023,42(10):1251-1264. DOI： 10.19969/j.fxcsxb.23033101.

摘要

建立了气相色谱-串联质谱（GC-MS/MS）同时测定三七中212种农药残留的方法。样品以乙腈-乙酸乙酯（体积比1∶3）提取，经 FaPEx-BKT50固相萃取柱净化，待测物采用GC-MS/MS测定，以保留时间和特征离子对定性，外标法定量。结果表明，212种农药在10~200 µg/L范围内线性关系良好，相关系数（,r,2,）均大于0.999，检出限（,S,/,N=,3）为0.003~0.02 mg/kg，定量下限（,S,/,N,=10）为0.01~0.05 mg/kg。在空白样品中进行0.02、0.04、0.1 mg/kg 3个水平的加标回收实验，212种农药的平均回收率为60.0%~121%，相对标准偏差（RSD）为0.90%~31%。该方法简单、灵敏、稳定，可有效去除复杂基体的干扰，各项技术指标均满足国内外农药残留的限量要求，适用于中药材三七中痕量农药的残留测定。

Abstract

A method for the simultaneous determination of 212 pesticide residues in ,Panax notoginseng, by gas chromatography-tandem mass spectrometry（GC-MS/MS） was developed. The samples were extracted by acetonitrile-ethyl acetate（1∶3，by volume），and cleaned up by a FaPEx-BKT50 solid phase extraction cartridge. The analytes were measured on GC-MS/MS，characterized by retention time and characteristic ion pairs，and quantified by external standard method. The results showed that the analytes were linear in the range of 10-200 µg/L，with linear correlation coefficients（,r,2,） more than 0.999.The limits of detection（,S,/,N=,3） for the analytes were 0.003-0.02 mg/kg and the limits of quantitation（,S,/,N=,10） were 0.01-0.05 mg/kg. At the spiked levels of 0.02，0.04，0.1 mg/kg，the average recoveries of 212 analytes in blank sample were 60.0%-121% with relative standard deviations（RSDs） of 0.90%-31%. The method is simple，sensitive and stable，and all the technical parameters meet the requirements of the domestic and international standards for pesticide residue monitoring，and can be applied to the determination of trace pesticide residues in ,Panax notoginseng,.

关键词

气相色谱-串联质谱法（GC-MS/MS）三七多农药残留

Keywords

gas chromatography-tandem mass spectrometry（GC-MS/MS）Panax notoginsengmulti-pesticide residues

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Hunan Provincial Key Laboratory of Food Science and Biotechnology，College of Food Science and Technology Hunan Agricultural University

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Simultaneous Determination of 212 Pesticide Residues in Panax Notoginseng by Gas Chromatography-Tandem Mass Spectrometry

DOI：10.19969/j.fxcsxb.23033101

摘要

Abstract

关键词

Keywords

references