Determination of Acrylamide in Honey Samples by Modified QuEChERS/Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

ZHANG Lin-yun; JI Wen-liang; SHEN Fei; WANG Xin-nan; ZHONG Cheng; ZHU Feng

doi:10.19969/j.fxcsxb.23060505

您当前的位置：

首页 >

文章列表页 >

Determination of Acrylamide in Honey Samples by Modified QuEChERS/Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

Scientific Papers | 更新时间：2023-10-18

- Determination of Acrylamide in Honey Samples by Modified QuEChERS/Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry
- Journal of Instrumental Analysis Vol. 42, Issue 10, Pages: 1272-1278(2023)
- 作者机构：
  
  1.江苏省疾病预防控制中心，江苏南京 210009
  2.沃特世科技（上海）有限公司，上海 200120
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.23060505
  CLC：
扫描看全文
张琳昀,吉文亮,沈菲等.改进的QuEChERS/超高效液相色谱-串联质谱法测定蜂蜜中的丙烯酰胺[J].分析测试学报,2023,42(10):1272-1278.

ZHANG Lin-yun,JI Wen-liang,SHEN Fei,et al.Determination of Acrylamide in Honey Samples by Modified QuEChERS/Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2023,42(10):1272-1278.
张琳昀,吉文亮,沈菲等.改进的QuEChERS/超高效液相色谱-串联质谱法测定蜂蜜中的丙烯酰胺[J].分析测试学报,2023,42(10):1272-1278. DOI： 10.19969/j.fxcsxb.23060505.

ZHANG Lin-yun,JI Wen-liang,SHEN Fei,et al.Determination of Acrylamide in Honey Samples by Modified QuEChERS/Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2023,42(10):1272-1278. DOI： 10.19969/j.fxcsxb.23060505.

摘要

基于改进的基质分散固相萃取（QuEChERS）法，建立了超高效液相色谱-串联质谱检测蜂蜜中丙烯酰胺的方法。蜂蜜样品经纯水溶解分散后，加入乙腈提取，采用QuEChERS法净化。以0.1%甲酸水溶液和甲醇为流动相梯度洗脱，丙烯酰胺经ZORBAX RRHD Eclipse Plus C,18,色谱柱（3.0 mm×150 mm，1.8 μm）分离，在电喷雾电离源（ESI）正离子模式下，采用多反应监测（MRM）模式进行检测，内标法定量。丙烯酰胺在2.0~500 ng/mL范围内线性关系良好，相关系数（,r,2,）为0.999 7，方法检出限（LOD）和定量下限（LOQ）分别为0.42 μg/kg和1.38 μg/kg。蜂蜜样品中，丙烯酰胺在低、中、高3个加标水平下的平均回收率为89.2%~96.3%，相对标准偏差（RSD，,n,=6）为2.2%~4.2%。采用建立的方法对30份市售蜂蜜进行测定，丙烯酰胺的检出率为100%，含量为2.5~40.4 μg/kg。该方法简单、快速，灵敏度高、准确性好，适用于蜂蜜中丙烯酰胺的测定。

Abstract

An analytical method for the determination of acrylamide in honey samples by modified QuEChERS using ultra high performance liquid chromatography-tandem mass spectrometry was established. After dissolved by water，honey samples was extracted by acetonitrile，and then the extracted supernatant was cleaned by matrix dispersion solid phase extraction. The target was separated by ZORBAX RRHD Eclipse Plus C,18, column（3.0 mm×150 mm，1.8 μm） using 0.1% formic acid solution and methanol as mobile phase by gradient elution，and analyzed in positive electrospray ionization mode by multiple reaction monitoring（MRM） mode. Acrylamide was quantified by internal standard method. The analyte showed a good relationship in concentration range of 2.0-500 ng/mL，with a correlation coefficient（,r,2,） of 0.999 7. The limits of detection and quantitation of the method was 0.42 μg/kg and 1.38 μg/kg，respectively. Average recoveries of acrylamide at low，medium and high spiked levels in honey matrices were 89.2%-96.3%，and relative standard deviations（RSDs，,n,=6） were 2.2%-4.2%. The proposed method was applied for the determination of acrylamide in 30 honey samples，and all samples showed positive results. The acrylamide contents of 30 honey samples ranged from 2.5 to 40.4 μg/kg. This method was suitable for analysis of acrylamide in honey samples with the advantages of simplicity，rapidity，high sensitivity and accuracy.

关键词

QuEChERS超高效液相色谱-串联质谱法蜂蜜丙烯酰胺

Keywords

QuEChERSultra high performance liquid chromatography-tandem mass spectrometryhoneyacrylamide

references

Wang A L，Wan X Z，Zhuang P，Jia W，Ao Y，Liu X H，Tian Y M，Zhu L，Huang Y Y，Yao J X，Wang B J，Wu Y Z，Xu Z S，Wang J Y，Yao W X，Jiao J J，Zhang Y. Proc. Natl. Acad. Sci. USA，2023，120（18）：1-12.

Ministry of Health. No. 4 Bulletin of the National Health Commission of the People's Republic of China（卫生部. 中华人民共和国卫生健康委员会第4号）. ［2005-04-13］. http：//www.nhc.gov.cn/sps/s7891/200804/4e977ac096354aacb777f169b05caa65.shtmlhttp://www.nhc.gov.cn/sps/s7891/200804/4e977ac096354aacb777f169b05caa65.shtml.

European Union. Commission Regulation（EU）2017/2158 of 20 November 2017 Establishing Mitigation Measures and Benchmark Levels for the Reduction of the Presence of Acrylamide in Food. Off. J. Eur. Union，2017，304：24–44.

South Korean Ministry of Food and Drug Safety. Recommended Specification Operation Plan for Acrylamide in Food. 2020，10：1-19.

Yoshioka T，Izumi Y，Nagatomi Y，Miyamoto Y，Suzuki K，Bamba T. Food Chem.，2019，294：486-492.

Xu R，Gao S Q，Wu Y Y，Mao Y C. J. Instrum. Anal.（徐蕊，高仕谦，吴友谊，毛宇成. 分析测试学报），2020，39（6）：722-728.

Altunay N，Elik A，Tuzen M，Lanjwani M F，Mogaddam M R A. J. Food Compos. Anal.，2023，115：1-7.

Wang M Y，Lu Y P，Liu S，Chen D. Food Sci. Technol.（王梦颖，卢跃鹏，刘嵩，陈丹. 食品科技），2019，44（3）：309-312.

Yin B. Guangzhou Chem. Ind.（殷斌. 广州化工），2022，50（10）：83-85.

Wang J L，Cai Z X，Zhang N G，Hu Z Y，Zhang J，Ying Y，Zhao Y X，Feng L，Zhang J S，Wu P G. Food Chem.，2022，388：1-7.

Petrarca M H，Rosa M A，Queiroz S C N，Godoy H T. J. Chromatogr. A，2017，1522：62-69.

Zhang D X，Luo C Y，Zhang Y M. Chem. World（张东霞，罗春阳，张永明. 化学世界），2020，61（7）：507-511.

Huang S，Lu S Y，Huang C S，Sheng J R，Zhang L X，Su W，Xiao Q. Sens. Actuators B，2016，224：22-30.

Sáez-Hernández R，Ruiz P，Mauri-Aucejo A R，Yusa V，Cervera M L. Food Control，2022，141：1-8.

Yin B，Wu Y Y，Zhou Z H，Zhou J W. J. Instrum. Anal.（殷斌，吴友谊，周震华，周靖雯. 分析测试学报），2017，36（2）：280-283.

GB 5009. 204-2014. Determination of Acrylamide in Food. National Standards of the People's Republic of China（食品中丙烯酰胺的测定. 中华人民共和国国家标准）.

Tölgyesi Á，Sharma V. J. Chromatogr. B，2020，1136：1-5.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Determination of Seven Lincosamides and Macrolides Residues in Honeys by High Performance Liquid Chromatography-Tandem Mass Spectrometry with ZnO QuEChERS

Simultaneous Determination of Tetrabromobisphenol A and Hexabromocyclododecane in Aquatic Products by QuEChERS Extraction/Ultra high Performance Liquid Chromatography-Tandem Mass Spectrometry

Residue Determination of Niclosamide-olamine in Twenty Two Kinds of Agricultural Products by UPLC-MS/MS

Simultaneous Determination of 16 Coumarins and Vanillins and Their Derivatives in Rice by QuEChERS-Isotope Internal Standard/Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

Related Author

No data

Related Institution

武汉食品化妆品检验所

环境保护部华南环境科学研究所国家环境保护环境污染健康风险评价重点实验室

长安大学环境科学与工程学院旱区地下水文与生态效应教育部重点实验室

锦州医科大学公共卫生学院

College of Horticulture and Landscape，Tianjin Agricultural University

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰