In this study，an ultra performance liquid chromatography-quadrupole-time-of-flight high-resolution mass spectrometry（UPLC-Q-TOF MS） method was developed for the rapid screening and quantitative analysis of 15 prostaglandin analogues in cosmetics. The analytes in sample were dispersed in saturated sodium chloride solution（wax samples were dispersed with tetrahydrofuran），then extracted with acetonitrile. After centrifugation and filtration，the UPLC separation of 15 prostaglandin analogues was performed on Poroshell 120 EC-C

18

column（150 mm×3.0 mm，2.7 μm），using 5 mmol/L ammonium formate（containing 0.1% formic acid） solution and acetonitrile as mobile phase by gradient elution. 15 prostaglandin analogues were detected by positive ion electrospray ionization（ESI） in the Targeted MS/MS mode. To construct a screening library with the software by the retention time，exact mass of primary parent ions，isotope abundance ratio and secondary sub-ion library matching were collected. The results of matching were used to rapid identification of 15 prostaglandin analogues in cosmetics. The quantification of primary parent ions was performed by external standard method. 15 prostaglandin analogues were all well

separated. The linear correlation coefficients（

r

2

） were more than 0.99. The limits of detection（LODs） ranged of 0.3-17.6 μg/g and the limits of quantitation（LOQs） ranged of 1.1-58.8 μg/g. The recoveries at three spiked levels in lotion，cream，emulsion，powder and gel matrices varied from 70.1% to 134%，with relative standard deviations not more than 7.5%. The established method is rapid，simple and accurate，which is suitable for the screening and determination of 15 prostaglandin analogues in cosmetics，providing technical support for cosmetic risk identification.