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1.河南农业大学 食品科学技术学院,河南 郑州 450000
2.河南九豫全食品有限公司,河南 新乡 453000
朱瑶迪,博士,副教授,研究方向:肉品加工与安全控制,食品微生物快速检测技术,食品、农产品无损检测技术研究,E-mail:zhu_yaodi@163.com
纸质出版日期:2024-12-15,
收稿日期:2024-03-13,
修回日期:2024-05-20,
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屠前程,田家齐,朱瑶迪,李苗云,赵莉君,刘世杰,梁栋.不同形状纳米银溶胶基底的增强特性比较及其对食源性芽孢的识别和定量研究[J].分析测试学报,2024,43(12):1919-1926.
TU Qian-cheng,TIAN Jia-qi,ZHU Yao-di,LI Miao-yun,ZHAO Li-jun,LIU Shi-jie,LIANG Dong.Comparison of Enhancement Properties of Different Shapes of Nanosilver Sol Substrates and Its Application in Identification and Quantification of Food-borne Spores[J].Journal of Instrumental Analysis,2024,43(12):1919-1926.
屠前程,田家齐,朱瑶迪,李苗云,赵莉君,刘世杰,梁栋.不同形状纳米银溶胶基底的增强特性比较及其对食源性芽孢的识别和定量研究[J].分析测试学报,2024,43(12):1919-1926. DOI: 10.12452/j.fxcsxb.24031302.
TU Qian-cheng,TIAN Jia-qi,ZHU Yao-di,LI Miao-yun,ZHAO Li-jun,LIU Shi-jie,LIANG Dong.Comparison of Enhancement Properties of Different Shapes of Nanosilver Sol Substrates and Its Application in Identification and Quantification of Food-borne Spores[J].Journal of Instrumental Analysis,2024,43(12):1919-1926. DOI: 10.12452/j.fxcsxb.24031302.
该文制备并考察了3种不同形貌纳米银溶胶基底对枯草芽孢杆菌、蜡样芽孢杆菌和产气荚膜梭菌的的增强效应,并选择增强效果最优的花状纳米银(AgNF)进行表面增强拉曼散射(SERS)分析。结果显示,吡啶二羧酸钙(Ca
2+
-DPA)在3种芽孢拉曼光谱中的出峰强度差异明显,是区别3种食源性芽孢的主要特征峰。基于SERS数据对上述3种芽孢的结构进行解析,并结合聚类分析法和K近邻算法实现了3种芽孢的快速识别。最后,通过构建枯草芽孢杆菌不同浓度对数与其1 537 cm
-1
处SERS峰强度之间线性关系的数字化定量方程,实现了食源性芽孢的快速定量。
In this paper,the enhancement effects of three kinds of nano-silver sol substrates with different morphologies on
Bacillus subtilis
,
Bacillus cereus
and
Clostridium perfringens
were prepared and investigated,and the flower-shaped nano-silver (AgNF) with the best enhancement effect was selected for surface enhanced Raman scattering (SERS) analysis. The results showed that the peak intensity of calcium pyridine dicarboxylate (Ca
2+
-DPA) in Raman spectra of three kinds of spores was obviously different,which was the main characteristic peak to distinguish three kinds of food-borne spores. Based on SERS data,the structures of the above three kinds of spores were analyzed,and the rapid identification of the three kinds of spores was realized by combining cluster analysis and K nearest neighbor algorithm. Finally,by constructing the digital quantitative equation of the linear relationship between the logarithm of different concentrations of
Bacillus subtilis
and the SERS peak intensity at 1 537 cm
-1
,the rapid quantification of food-borne spores was realized.
表面增强拉曼散射食源性芽孢不同形状的银纳米颗粒快速识别数字化定量
surface enhanced Raman scatteringfood-borne sporesdifferent shapes AgNPsrapid identificationdigital quantification
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