A two dimensional photonic crystal molecularly imprinted hydrogel(MIPs) sensor with specific recognition to glycoproteins was prepared by using concanavalin A(Con A) as the imprinted molecule

methylidenebisacrylamide(MBA) as the cross linking agents

acrylamide(AAm) and 4 vinylbenzeneboronic acid as the functional monomers

tetramethylethylenediamine(TEMED) as an initiator and ammonium persulfate(APS) as a catalyst.Polymerization was carried out to form a Con A imprinted hydrogel on the surface of two dimensional photonic crystal.After elution with 10% acetic acid solution

a MIPs sensor for specific recognition to Con A was constructed.Results showed that the diameter of Debye ring changed most significantly

and the target object was the most sensitive to be identified when the cross linking degree of MIPs was 10%.The sensor could be used in the enrichment and real time detection of target molecules as it had a good response to Con A in the concentration range of 0-05 mg/mL.Moreover

the introduction of phenyl boronic acid increased the recognition sites of the imprinted cavities

and further improved the specific recognition of the MIPs toward the carbohydrate molecules.The hydrogel sensor presented a good selectivity when binding to the proteins in ovalbumin(OVA)

bovine hemoglobin(BHb)

lysozyme(Lyz)

trypsin(Try) solution under the same concentration.This method is easy to operate

low cost

reusable and needless for any special equipments.Furthermore

it could be extended to the real time and fast detection for other glycoproteins.