Determination of Eight Alkaloids in Cynoglossum amabile Stapf et Drumm by Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry with Aqueous Two phase Extraction

樊轻亚; 许卫军; 代春美

doi:doi：10.3969/j.issn.1004-4957.2019.11.007

您当前的位置：

首页 >

文章列表页 >

Determination of Eight Alkaloids in Cynoglossum amabile Stapf et Drumm by Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry with Aqueous Two phase Extraction

- Determination of Eight Alkaloids in Cynoglossum amabile Stapf et Drumm by Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry with Aqueous Two phase Extraction
- Vol. 38, Issue 11, Pages: 1328-1334(2019)
- 作者机构：
  
  1. 信阳职业技术学院药学院
  2. 中国中医科学院
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2019.11.007
  CLC：
- Published：2019
- 稿件说明：
移动端阅览
樊轻亚, 许卫军, 代春美. Determination of Eight Alkaloids in Cynoglossum amabile Stapf et Drumm by Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry with Aqueous Two phase Extraction[J]. 2019, 38(11): 1328-1334.
DOI：

樊轻亚, 许卫军, 代春美. Determination of Eight Alkaloids in Cynoglossum amabile Stapf et Drumm by Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry with Aqueous Two phase Extraction[J]. 2019, 38(11): 1328-1334. DOI： doi：10.3969/j.issn.1004-4957.2019.11.007.

摘要

建立了乙醇-磷酸氢二钾双水相萃取技术结合超高效液相色谱-串联质谱法(UHPLC-MS/MS)同时测定倒提壶中8种生物碱含量的方法。采用单因素实验考察了双水相的萃取条件对倒提壶中8种生物碱萃取率的影响，最佳萃取条件为：乙醇质量分数为30%，K2HPO4·3H2O的质量分数为20%，萃取剂与样品相比为6∶1，水相的pH值为4.0。以乙腈-0.1 mol/L醋酸铵为流动相进行UHPLC-MS/MS分析，8种生物碱之间具有良好的分离度，各成分在一定质量浓度范围内具有良好的线性关系(r2＞0.99)。方法的精密度、重复性及加标回收率的相对标准偏差（RSD）均小于 3.0%。方法简单快捷、灵敏准确，可满足倒提壶中生物碱检测的要求。

Abstract

An ultrahigh performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS) with EtOH/K2HPO4·3H2O aqueous two-phase extraction was developed for the simultaneous determination of eight alkaloids in Cynoglossum amabile Stapf et Drumm.Effects of aqueous two phase extraction conditions on extraction efficiencies of eight alkaloids were optimized by single factor experiment.The optimum conditions were as follows:30%(mass ratio) of ethanol

20%(mass ratio) of K2HPO4·3H2O

6∶1 for an extractant to sample ratio

and 4.0 for pH value.The extract was analyzed by UHPLC-MS/MS with acetonitrile-0.1 mol/L ammonium acetate as mobile phases

and eight alkaloids in the extract were well separated.There were good linear relationships for the analytes in the certain concentration ranges with their correlation coefficients(r2) larger than 0.99.The relative standard deviations(RSDs) for precision

reproducibility and recovery were all less than 3.0%.The method is convenient，sensitive and accurate，and is suitable for the determination and confirmation of eight alkaloids in Cynoglossum amabile Stapf et Drumm.

关键词

Keywords

references

Views

222

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on Chemical Differences of Alkaloids from Fritillariae Cirrhosae Bulbus and Their Counterfeits Based on Liquid Chromatography-Mass Spectrometry Technology

Research Advances of Alkaloid Active Ingredients in Kratom

Determination of Six Alkaloids in Ervatamia Officinalis Tsiang by Ultra High Performance Liquid Chromatography－Tandem Mass Spectrometry with Dispersive Solid Phase Extraction

Lipid Interference in Pyrolysis-Gas Chromatography-Mass Spectrometry Detection of Microplastics：Problem Identification，Validation Approaches，and Mitigation Strategies

Progress in Analytical Methods for Phthalate Plasticizers in （Micro）plastic Release Systems and Environmental Media

Related Author

ZHANG Mei

WANG Wei

QIAN Chong

XU Shuang-shuang

XIANG Shu-qin

MA Jun-jun

YANG Hui-ying

NIU Chuang

Related Institution

Beijing Key Laboratory of Organic Materials Testing Technology & Quality Evaluation，Institute of Analysis and Testing，Beijing Academy of Salience and Technology（Beijing Center for Physical and Chemical Analysis）

Beijing Health Vocational College

School of Biomedicine，Beijing City University

National Anti-Drug Laboratory Beijing Regional Center

CAAC Central and Southern Regional Administration

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
Total Visits：942211

⁰