Investigation on Immunomodulatory Effect of Low Molecular Weight Seleno aminopolysaccharide on Acathopagrus Schlegelii by Spleen Metabonomics Analysis

周秀锦; 张静; 邵宏宏; 相兴伟; 冷向阳; 杨会成

doi:doi：10.3969/j.issn.1004-4957.2019.10.001

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Investigation on Immunomodulatory Effect of Low Molecular Weight Seleno aminopolysaccharide on Acathopagrus Schlegelii by Spleen Metabonomics Analysis

- Investigation on Immunomodulatory Effect of Low Molecular Weight Seleno aminopolysaccharide on Acathopagrus Schlegelii by Spleen Metabonomics Analysis
- Vol. 38, Issue 10, Pages: 1155-1162(2019)
- 作者机构：
  
  1. 舟山海关综合技术服务中心
  2. 浙江海洋开发研究院
  3. SCIEX公司
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2019.10.001
  CLC：
- Published：2019
- 稿件说明：
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周秀锦, 张静, 邵宏宏, 相兴伟, 冷向阳, 杨会成. Investigation on Immunomodulatory Effect of Low Molecular Weight Seleno aminopolysaccharide on Acathopagrus Schlegelii by Spleen Metabonomics Analysis[J]. 2019, 38(10): 1155-1162.
DOI：

周秀锦, 张静, 邵宏宏, 相兴伟, 冷向阳, 杨会成. Investigation on Immunomodulatory Effect of Low Molecular Weight Seleno aminopolysaccharide on Acathopagrus Schlegelii by Spleen Metabonomics Analysis[J]. 2019, 38(10): 1155-1162. DOI： doi：10.3969/j.issn.1004-4957.2019.10.001.

摘要

以冷冻甲醇提取，C18色谱柱和HILIC色谱柱分别分离黑鲷脾脏中的内源性代谢物，采用基于超高效液相色谱-飞行时间质谱联用技术(UPLC-TOF-MS)的非靶向代谢组学研究方法，分析了黑鲷饲喂低聚硒化氨基多糖后脾脏中内源性代谢物的变化差异，揭示了低聚硒化氨基多糖调节黑鲷免疫功能的潜在机制。采用XCMSplus软件结合高分辨二级质谱数据库处理质谱原始数据，筛选出潜在生物标志物，并通过Metabo Analyst 4.0网站分析相关代谢通路。结果表明，黑鲷饲喂低聚硒化氨基多糖后脾脏中的36个代谢物发生显著变化；低聚硒化氨基多糖可通过9条代谢通路增强黑鲷的免疫机能。该研究结果为阐明低聚硒化氨基多糖的免疫增强机制提供了科学依据。

Abstract

To investigate the potential immunomodulatory mechanism of low molecular weight seleno aminopolysaccharide，an untargeted metabonomics method based on ultra high performance liquid chromatography-time of flight mass spectrometry(UPLC-TOF-MS) was developed for analysis of the variations of spleen endogenous metabolites in Acathopagrus schlegelii after they were extracted with frozen methanol

then isolated with a C18 column and a HILIC column

respectively.The potential biomarkers were screened out by non-targeted mass spectrometry with XCMSplus software and Metabolite HR MS2 library

and the related metabolic pathways were analyzed by Metabo Analyst 4.0 website.Results showed that the spleen metabolites in the group fed with the low molecular weight seleno aminopolysaccharide were significantly different from those in the blank group

and 36 biomarkers were found and identified.Low molecular weight seleno-aminopolysaccharide could enhance the immune function of Acathopagrus schlegelii through 9 metabolic pathways.This study provides a scientific basis for elucidating the immunoenhancement mechanism of low molecular weight seleno aminopolysaccharide.

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