Determination of Histamine in Fish Samples Based on Colloidal Gold Modified Electrode

周婵媛; 赵晓娟; 王春利; 曾晓房; 白卫东

doi:doi：10.3969/j.issn.1004-4957.2019.02.017

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Determination of Histamine in Fish Samples Based on Colloidal Gold Modified Electrode

更新时间：2023-04-18

- Determination of Histamine in Fish Samples Based on Colloidal Gold Modified Electrode
- Vol. 38, Issue 2, Pages: 229-234(2019)
- 作者机构：
  
  1. 仲恺农业工程学院轻工食品学院
  2. 中华人民共和国江门海关
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2019.02.017
  CLC：
- Published：2019
- 稿件说明：
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周婵媛, 赵晓娟, 王春利, 曾晓房, 白卫东. Determination of Histamine in Fish Samples Based on Colloidal Gold Modified Electrode[J]. 2019, 38(2): 229-234.
DOI：

周婵媛, 赵晓娟, 王春利, 曾晓房, 白卫东. Determination of Histamine in Fish Samples Based on Colloidal Gold Modified Electrode[J]. 2019, 38(2): 229-234. DOI： doi：10.3969/j.issn.1004-4957.2019.02.017.

摘要

基于胶体金修饰的玻碳电极，利用电流~时间曲线法建立了一种简便、灵敏的组胺检测方法。优化了底液的pH值和组胺的电化学测试方法及条件，考察了修饰电极的电化学性能。结果表明，组胺在胶体金修饰电极上的响应电流(-I

μA)与其浓度(c

μmol/L)在0.1~64 μmol/L范围内呈良好的线性关系，检出限为0.033 μmol/L，在带鱼和黄花鱼样品中的加标回收率分别为94.4%~106%、91.8%~106%，相对标准偏差分别为3.2%、2.5%。该法操作简单、检测速度快、成本低，适用于带鱼和黄花鱼等鱼样中组胺的测定。

Abstract

A simple and sensitive method was established for the detection of histamine by using the current-time curve based on a colloidal gold modified glass carbon electrode(AuCS/GCE).The electrochemical properties of the modified electrode were investigated.The pH value of the base solution

and the electrochemical detection methods and conditions for histamine were optimized.Results showed that there was a good linear relationship for the response current(-I

μA) of histamine on the AuCS/GCE in the concentration(c

μmol/L) range of 0.1-64 μmol/L

with the detection limit of 0.033 μmol/L.The recoveries for histamine in the hairtail and yellow croaker samples were in the ranges of 94.4%-106% and 91.8%-106%

respectively.The method was simple

rapid and low cost

and was suitable for the determination of histamine in food samples such as hairtail and yellow croaker.

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