Study on “Quality－Quantity” Double Standards Quality Analysis Method for Traditional Chinese Medicine <italic style="font-style: italic">Scutellaria Baicalensis</italic> Georgi Based on Dual Wavelength Equal Baseline Differential Fusion Spectrum

ZHANG Qiang; LUO Xi; LI Tian-jiao; WANG Shuai; BAO Yong-rui; GUAN Xin; MENG Xian-sheng

doi:10.19969/j.fxcsxb.23051402

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Study on “Quality－Quantity” Double Standards Quality Analysis Method for Traditional Chinese Medicine Scutellaria Baicalensis Georgi Based on Dual Wavelength Equal Baseline Differential Fusion Spectrum

Scientific Papers | 更新时间：2023-10-24

- Study on “Quality－Quantity” Double Standards Quality Analysis Method for Traditional Chinese Medicine Scutellaria Baicalensis Georgi Based on Dual Wavelength Equal Baseline Differential Fusion Spectrum
- Journal of Instrumental Analysis Vol. 42, Issue 8, Pages: 968-975(2023)
- 作者机构：
  
  1.辽宁中医药大学药学院，辽宁大连 116600
  2.辽宁省中药多维分析专业创新技术中心，辽宁大连 116600
  3.辽宁省现代中药研究工程实验室，辽宁大连 116600
  4.中国人民解放军北部战区总医院肾脏病科，辽宁沈阳 110016
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.23051402
  CLC：
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张强,罗曦,李天娇等.基于双波长等基线差示融合图谱的中药黄芩“质－量”双标质量分析方法研究[J].分析测试学报,2023,42(08):968-975.

ZHANG Qiang,LUO Xi,LI Tian-jiao,et al.Study on “Quality－Quantity” Double Standards Quality Analysis Method for Traditional Chinese Medicine Scutellaria Baicalensis Georgi Based on Dual Wavelength Equal Baseline Differential Fusion Spectrum[J].Journal of Instrumental Analysis,2023,42(08):968-975.
张强,罗曦,李天娇等.基于双波长等基线差示融合图谱的中药黄芩“质－量”双标质量分析方法研究[J].分析测试学报,2023,42(08):968-975. DOI： 10.19969/j.fxcsxb.23051402.

ZHANG Qiang,LUO Xi,LI Tian-jiao,et al.Study on “Quality－Quantity” Double Standards Quality Analysis Method for Traditional Chinese Medicine Scutellaria Baicalensis Georgi Based on Dual Wavelength Equal Baseline Differential Fusion Spectrum[J].Journal of Instrumental Analysis,2023,42(08):968-975. DOI： 10.19969/j.fxcsxb.23051402.

摘要

建立了以对照药材为基准物质的定性和不依赖多种对照品定量的黄芩药材“质－量”双标控制方法。采用高效液相色谱（HPLC）技术，以对照药材为基准物质建立了黄芩特征图谱，通过鉴定特征峰的化学成分，共确定了11个共有特征峰，通过质谱鉴定出黄岑药材的8个成分，经对照品比对，指认出共有特征峰中的4个化学成分，分别为黄芩苷、汉黄芩苷、黄芩素以及汉黄芩素。通过对照药材和供试药材特征峰的相似度，可明确药材真伪，即“质”，结果显示各批次供试药材与黄芩对照药材的相似度均大于0.990；对内标成分“黄芩苷”进行准确定量，以内标成分计算不同批次供试品中各特征峰的相对含量，取“平均数－标准差”作为特征峰化学成分相对于内标物质化学成分的相对含量下限，依据特征峰相对含量下限明确黄芩药材优劣，即“量”。该方法不依赖多种对照品，能清晰、快速地判断药材的真伪优劣，可为黄芩的质量控制方法提供参考。

Abstract

A qualitative and quantitative double standards control method for ,Scutellaria baicalensis ,Georgi was established，which takes the reference traditional Chinese medicinal materials as the primary standard but does not rely on the quantification of multiple reference materials．The characteristic chromatogram for ,Scutellaria baicalensis ,Georgi was established by using HPLC technique and the reference traditional Chinese medicinal materials as the primary standard．The chemical components of the characteristic peaks were identified by Q－TOF MS．A total of 11 common characteristic peaks were indentified，and 8 components of ,Scutellaria Baicalensis, Georgi were identified through MS identification．After comparison with reference materials，four chemical components were identified among the common characteristic peaks，namely baicalin，wogonoside，baicalein and wogonin．Based on the similarity of characteristic peaks between the control and test medicinal materials，the authenticity of the medicinal materials，i.e. “quality”，was determined．The similarities between each batch of test medicinal materials and the control medicinal materials of ,Scutellaria baicalensis ,Georgi were greater than 0.990．The internal standard component “baicalin” was accurately quantified，which was used to calculate the relative content of each characteristic peak in different batches of test samples．The “value of subtracting the standard deviation from the mean” was taken as the lower limit for the relative content of the chemical components of the characteristic peak relative to the chemical components of the internal standard substance．The advantages and disadvantages of ,Scutellaria baicalensis ,Georgi were determined according to the lower limit for the relative content of the characteristic peak，that is，the “quantity”．This method could be used to clearly and quickly determine the authenticity of medicinal materials without any reliance on multiple reference materials，providing a reference for the quality control methods for scutellaria baicalensis.

关键词

黄芩对照药材质量控制特征图谱黄芩苷双波长等基线差示融合

Keywords

Scutellaria baicalensis Georgireference traditional Chinese medicinal materialsquality controlspecific chromatogrambaicalindual wavelength equal baseline differential fusion

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Study on “Quality－Quantity” Double Standards Quality Analysis Method for Traditional Chinese Medicine Scutellaria Baicalensis Georgi Based on Dual Wavelength Equal Baseline Differential Fusion Spectrum

DOI：10.19969/j.fxcsxb.23051402

摘要

Abstract

关键词

Keywords

references