A large number of ,β,2,-agonist poisoning or death cases have been reported. It is urgent to establish a toxicological assay for ,β,2,-agonist poisoning in blood applicable to forensic toxicology analysis since the current methods for ,β,2,-agonists detection mainly focus on the edible tissues of animal products or the illegal addition of drugs．Compared with traditional whole blood collection techniques，the dried blood spots（DBSs） offers several advantages. It is a simple and minimally invasive sample collection technique，involving collecting a small sample volume，easy to transport and store．In recent years，the DBSs have been gradually applied to drug metabolism and toxicological analysis，and a large number of quantitative methods for the detection of drug abuse and psychoactive substances have been reported．An ultra-high performance liquid chromatography－tandem mass spectrometry（UPLC－MS/MS） was developed for the qualitative and quantitative determination of 29 ,β,2,-agonists in dried blood spots in this paper．The mobile phase，type and proportion of extraction solvents were optimized. Stability of 29 ,β,2,-agonists in the DBS sample was investigated. The DBSs were firstly extracted with methanol， then separated on a Phenomenex Kinetex F5（3.0 mm × 100 mm，2.6 μm） column，with 1 mmol/L ammonium formate－0.01% formic acid aqueous solution and acetonitrile as mobile phases，and finally analyzed by UPLC－MS/MS．Results showed that there were good relationships for 29 ,β,2,-agonists in DBSs in a certain concentration range， with their correlation coefficients（,r,2,） of 0.995 8－0.999 9 and limits of detection（LOD） of 1－10 ng·mL,－1,. The spiked recoveries for 28 ,β,2,-agonists were in the range of 60.9%－124% except fenoterol，with relative standard deviations（RSD） less than 20%．The analytes in DBSs stored at －20 ℃ for 8 weeks showed the same stability as those of the whole blood sample．The method was accurate and simple，and was convenient to storage and transport．It provided a new method and thought for forensic toxicological analysis.