Fluorescent Probe for Detection of <italic style="font-style: italic">α</italic>-Glucosidase Activity Based on N-Doped Ti<sub>3</sub>C<sub>2</sub> MXene Quantum Dots

LI Jun; YANG Xin-jie; LUO Yan; LI Quan; ZONG Yu-hong; ZHANG Yan-li; WANG Hong-bin; YANG Wen-rong; PANG Peng-fei

doi:10.12452/j.fxcsxb.24010503

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Fluorescent Probe for Detection of α-Glucosidase Activity Based on N-Doped Ti₃C₂ MXene Quantum Dots

Scientific Papers | 更新时间：2024-08-16

- Fluorescent Probe for Detection of α-Glucosidase Activity Based on N-Doped Ti₃C₂ MXene Quantum Dots
- Journal of Instrumental Analysis Vol. 43, Issue 5, Pages: 674-681(2024)
- 作者机构：
  
  1.云南民族大学化学与环境学院，云南省教育厅环境功能材料重点实验室，云南昆明 650504
  2.迪肯大学生命与环境科学学院，澳大利亚维多利亚州吉朗 3217
- 作者简介：
- 基金信息：
- DOI：10.12452/j.fxcsxb.24010503
  CLC： O657.3;O629.8
- Published：15 May 2024，
  
  Received：05 January 2024，
  
  Revised：18 February 2024，
- 稿件说明：
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李军,杨新杰,罗焰,李泉,宗玉红,张艳丽,王红斌,杨文荣,庞鹏飞.氮掺杂Ti₃C₂ MXene量子点荧光探针用于α-葡萄糖苷酶活性检测[J].分析测试学报,2024,43(05):674-681.

LI Jun,YANG Xin-jie,LUO Yan,LI Quan,ZONG Yu-hong,ZHANG Yan-li,WANG Hong-bin,YANG Wen-rong,PANG Peng-fei.Fluorescent Probe for Detection of α-Glucosidase Activity Based on N-Doped Ti₃C₂ MXene Quantum Dots[J].Journal of Instrumental Analysis,2024,43(05):674-681.
李军,杨新杰,罗焰,李泉,宗玉红,张艳丽,王红斌,杨文荣,庞鹏飞.氮掺杂Ti₃C₂ MXene量子点荧光探针用于α-葡萄糖苷酶活性检测[J].分析测试学报,2024,43(05):674-681. DOI： 10.12452/j.fxcsxb.24010503.

LI Jun,YANG Xin-jie,LUO Yan,LI Quan,ZONG Yu-hong,ZHANG Yan-li,WANG Hong-bin,YANG Wen-rong,PANG Peng-fei.Fluorescent Probe for Detection of α-Glucosidase Activity Based on N-Doped Ti₃C₂ MXene Quantum Dots[J].Journal of Instrumental Analysis,2024,43(05):674-681. DOI： 10.12452/j.fxcsxb.24010503.

摘要

-葡萄糖苷酶是生物体糖代谢途径中不可或缺的一类酶，发展简单、灵敏、准确的

-葡萄糖苷酶活性检测及抑制剂筛选方法对于糖尿病的治疗与预防具有重要意义。该文基于氮掺杂Ti

MXene量子点（N-Ti

MQDs）荧光探针和内滤效应（IFE），构建了一种“开-关-开”型荧光传感

-葡萄糖苷酶活性检测及抑制剂筛选的新方法。研究发现，N-Ti

MQDs发射蓝色荧光（

= 440 nm），荧光量子产率为15.7%。其检测机理为：

-葡萄糖苷酶水解底物对硝基苯基-

-D-吡喃葡萄糖苷，水解产物对硝基苯酚通过内滤效应导致N-Ti

MQDs荧光猝灭；而抑制剂阿卡波糖可使

-葡萄糖苷酶的活性受到抑制，水解产物减少，N-Ti

MQDs荧光恢复。结果显示，N-Ti

MQDs探针荧光强度与

-葡萄糖苷酶浓度在5~300 U/L范围内呈良好线性关系，检出限为2.5 U/L（

= 3），对阿卡波糖的半最大抑制浓度（IC

）为178.5 μmol/L。该方法具有成本低、操作简单、灵敏度高、选择性好等特点，已成功用于人血清中

-葡萄糖苷酶活性的测定。

Abstract

-Glucosidase is an indispensable enzyme in the glucose metabolism pathway of organisms. The development of simple，sensitive and accurate detection methods for

-glucosidase activity and inhibitors screening are of great significance for the treatment and prevention of diabetes. In this work，a novel fluorescent "on-off-on" method for detection of

-glucosidase activity and its inhibitors screening was developed based on N-doped Ti

MXene quantum dots（N-Ti

MQDs） and internal filtration ef

fect（IFE）. The prepared N-Ti

MQDs emitted blue fluorescence（

= 440 nm） with a fluorescence quantum yield of 15.7%.

-Glucosidase can hydrolyze substrate

-nitrophenyl-

-D-glucopyranoside to produce

-nitrophenol，which causes fluorescence quenching of the N-Ti

MQDs through IFE. After adding the acarbose inhibitor，the activity of

-glucosidase is inhibited and the produced

-nitrophenol by hydrolysis is reduced，resulting in fluorescence recovery of N-Ti

MQDs. Fluorescent intensity of N-Ti

MQDs probe is proportional to

-glucosidase concentration in the range of 5-300 U/L，with a detection limit of 2.5 U/L（

S/N

= 3）. The half-maximal inhibitory concentration of inhibitor（IC

） value is calculated to be 178.5 μmol/L for acarbose. This proposed method has the advantages of low cost，simple operation，high sensitivity，and good selectivity，which has been successfully used for determination of

-glucosidase activity in human serum.

关键词

α-葡萄糖苷酶氮掺杂Ti3C2 MXene量子点荧光探针

Keywords

α-glucosidaseN-doped Ti3C2 MXenequantum dotsfluorescent probe

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⁰

Fluorescent Probe for Detection of α-Glucosidase Activity Based on N-Doped Ti3C2 MXene Quantum Dots

DOI：10.12452/j.fxcsxb.24010503

摘要

Abstract

关键词

Keywords

references

Fluorescent Probe for Detection of α-Glucosidase Activity Based on N-Doped Ti₃C₂ MXene Quantum Dots