Determination of 67 Indicative Components from Plant Extracts in Cosmetics by Ultra High Performance Liquid Chromatography- Tandem Mass Spectrometry

ZHANG Qiu-yan; HUANG Fang; LIANG Wei-wei; LIAO Jun-tao; WU Hui-qin; LUO Hui-tai

doi:10.12452/j.fxcsxb.24010601

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Determination of 67 Indicative Components from Plant Extracts in Cosmetics by Ultra High Performance Liquid Chromatography- Tandem Mass Spectrometry

Scientific Papers | 更新时间：2024-08-16

- Determination of 67 Indicative Components from Plant Extracts in Cosmetics by Ultra High Performance Liquid Chromatography- Tandem Mass Spectrometry
- Journal of Instrumental Analysis Vol. 43, Issue 5, Pages: 663-673(2024)
- 作者机构：
  
  广东省科学院测试分析研究所（中国广州分析测试中心），广东省化学测量与应急检测技术重点实验室，广东省中药质量安全工程技术研究中心，广东广州 510070
- 作者简介：
- 基金信息：
- DOI：10.12452/j.fxcsxb.24010601
  CLC： O657.7;TQ658
- Published：15 May 2024，
  
  Received：06 January 2024，
  
  Revised：28 February 2024，
- 稿件说明：
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张秋炎,黄芳,梁维维,廖均涛,吴惠勤,罗辉泰.UHPLC-MS/MS测定化妆品中67种植物提取物标识成分[J].分析测试学报,2024,43(05):663-673.

ZHANG Qiu-yan,HUANG Fang,LIANG Wei-wei,LIAO Jun-tao,WU Hui-qin,LUO Hui-tai.Determination of 67 Indicative Components from Plant Extracts in Cosmetics by Ultra High Performance Liquid Chromatography- Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2024,43(05):663-673.
张秋炎,黄芳,梁维维,廖均涛,吴惠勤,罗辉泰.UHPLC-MS/MS测定化妆品中67种植物提取物标识成分[J].分析测试学报,2024,43(05):663-673. DOI： 10.12452/j.fxcsxb.24010601.

ZHANG Qiu-yan,HUANG Fang,LIANG Wei-wei,LIAO Jun-tao,WU Hui-qin,LUO Hui-tai.Determination of 67 Indicative Components from Plant Extracts in Cosmetics by Ultra High Performance Liquid Chromatography- Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2024,43(05):663-673. DOI： 10.12452/j.fxcsxb.24010601.

摘要

建立了超高效液相色谱-串联质谱（UHPLC-MS/MS）测定化妆品中67种植物提取物标识成分的方法。化妆品样品经甲醇（含1%甲酸）超声提取，样液在0.1%甲酸溶液-乙腈流动相体系下经Agilent RRHD Eclipse Plus Zorbax C

（3.0 mm×100 mm，1.8 μm）色谱柱梯度洗脱分离。采用电喷雾正、负离子模式分别对67种成分进行定性定量分析，其中正离子采用多反应监测（MRM）方式，负离子采用动态多反应监测（DMRM）方式，以基质匹配外标法定量。以膏霜、乳液基质为代表，67种成分在各自质量浓度范围内线性关系良好，相关系数（

）均大于0.99；检出限为0.002 6~1.7 μg/g，定量下限为0.008 0~5.0 μg/g；3个不同加标水平下，膏霜、乳液基质的平均回收率分别为85.6%~117%和82.7%~116%，相对标准偏差（RSD，

=6）分别为1.0%~12%和0.90%~12%。该法简单快速，灵敏度高，适用于化妆品中67种植物提取物标识成分的定性鉴定和含量测定。

Abstract

A new method for the simultaneous and rapid determination of 67 indicative components from plant extracts in cosmetics by ultra high performance liquid chromatography-tandem mass spectrometry（UHPLC-MS/MS） was established. The cosmetic samples were dispersed with methanol（1% formic acid） and ultrasonic extraction. The filtrate was analyzed by a Agi

lent RRHD Eclipse Plus Zorbax C

chromatographic column（3.0 mm×100 mm，1.8 μm） with mobile phase consisted of 0.1% formic acid aqueous solution and acetonitrile. 67 indicative components from plant extracts were analyzed in the electrospray ionization source，including positive ion scanning with multiple reaction monitoring mode（MRM） and negative ion scanning with dynamic multiple reaction monitoring mode（DMRM） respectively. Creams and lotions were choosed as the representative matrix. The 67 indicative components showed good linearity with the correlation coefficients（

） greater than 0.99 in their respective mass concentration ranges. The detection limits（LODs） of 67 indicative components were in the range of 0.002 6-1.7 μg/g，and the quantitation limits（LOQs） were in the range of 0.008 0-5.0 μg/g. For cream and lotion samples，the average recoveries of 67 indicative components at three different concentration levels ranged of 85.6%-117% and 82.7%-116%，respectively； with relative standard deviations（RSDs，

=6） ranged of 1.0%-12% and 0.90%-12%. The established method is simple，rapid，effective，high sensitive，and is suitable for the qualitative identification and content determination of 67 indicative components from plant extracts in cosmetics.

关键词

超高效液相色谱-串联质谱法化妆品植物提取物标识成分

Keywords

UHPLC-MS/MScosmeticsplant extractsindicative components

references

Yuan Y Y，Qiao Y S，Dong Y L，Sun L. China Food Drug Admin. Mag. 袁莹莹，乔亚森，董亚蕾，孙磊. 中国食品药品监管），2022，（10）：64-71.

Zhang J Y，Li J，Zhang R，Qu C B. Chem. Eng. Des. Commun.（张静怡，李静，张蕊，曲春波. 化工设计通讯），2023，49（9）：91-93，96.

Hu J D，Wang H R. Flavour Fragrance Cosmet. （胡积东，王海瑞. 香料香精化妆品），2023，（3）：121-124.

Li G P，Meng X S，Ma L，Chen M，Lian X H，Bai H，Lei H M，Ma Q. J. Instrum. Anal.（李国萍，孟宪双，马麟，陈萌，连显会，白桦，雷海民，马强. 分析测试学报），2019，38（12）：1516-1525.

Tan J H，Li H Y，Xi S F，Guo C H，Wang J C，Xiong X T，Xian Y P，Guo X D. Chin. J. Anal. Chem.（谭建华，李慧勇，席绍峰，郭长虹，王继才，熊小婷，冼燕萍，郭新东. 分析化学），2015，43（1）：110-114.

Wang L，Zeng G F，Hou Y Y，Dong J，Cai C L，Xiao K Y，Zhang X C，Li J Y，Xie J J. J. Instrum. Anal.（王璐，曾广丰，侯颖烨，董洁，蔡翠玲，肖可茵，张宪臣，李嘉仪，谢建军. 分析测试学报），2023，42（9）：1085-1095.

Liang Z Y，Zhang Q Y，Zhou X，Kang H T，Liang Y R，Xuan S X，Wu H Q，Huang F，Chen G Q，Li Y J，Luo H T. J. Instrum. Anal.（梁梓洋，张秋炎，周熙，康怀腾，梁韵蕊，轩申鑫，吴惠勤，黄芳，陈桂琴，李杨杰，罗辉泰. 分析测试学报），2023，42（11）：1413-1423.

Gao X Y，Wang H Y，Liu Z M. Chin. Wild Plant Res.（高欣妍，王海英，刘志明. 中国野生植物资源），2018，37（4）：25-28.

Wu X Y，Li S L，Diao F Y，Li Q Y，Ran J F，Liu C L. J. Pharm. Res.（吴晓云，李思龙，刁飞燕，李启艳，冉金凤，刘春霖. 药学研究），2023，42（9）：680-684.

Fang J H，Zhu X Z，Wu Z. Flavour Fragrance Cosmet. （方继辉，朱新州，吴震. 香料香精化妆品），2013，（1）：25-27.

Xi S F，Li H Y，Tan J H，Xu H H，Wang J C，Xiong X T，Zhao T T，Xian Y P，Guo X D. J. Chin. Mass Spectrom. Soc.（席绍峰，李慧勇，谭建华，徐汉虹，王继才，熊小婷，赵田甜，冼燕萍，郭新东. 质谱学报），2015，36（4）：341-349.

Huang F，Wang Y Q，Huang X L，Luo H T，Lin X S，Zhu Z X，Deng X，Chen C，Wu H Q. Flavour Fragrance Cosmet. （黄芳，王玉芹，黄晓兰，罗辉泰，林晓珊，朱志鑫，邓欣，陈超，吴惠勤. 香料香精化妆品），2019，（3）：33-39.

Yuan Y Y，Dong Y L，Qiao Y S，Sun L，Shang J. Chin. J. Anal. Chem.（袁莹莹，董亚蕾，乔亚森，孙磊，尚靖. 分析化学），2022，50（12）：1899-1914.

Xiong C S H，Ding T M，Liu J，Yi O，Ding X P，Xie Y. Chin. J. Chromatogr.（熊陈思慧，定天明，刘杰，易鸥，丁晓萍，谢云. 色谱），2022，40（9）：817-824.

Zhang W Q，Hou L N，Li B，Liu H J，Wang H Y. China Surfactant Deterg. Cosmet.（张伟清，侯俐南，李彬，刘慧锦，王海燕. 日用化学工业），2021，51（6）：577-582.

Chinese Phamacopoeia Commission. Phamacopoeia of the People's Repubic of China. Beijing：China Medical Science Press（中国药典委员会. 中国药典. 北京：中国医药科技出版社），2020.

EC. Commission Decision 2002/657/EC of 12 August 2002 Implementing Council Directive 96/23/EC Concerning the Performance of Analytical Methods and the Interpretation of Results. Offic. J. Eur. Comm.，2002. ［2002-08-17］. http：//eur-lex.europa.eu/legal-content/EN/ALL/？uri=CELEX：32002D0657http://eur-lex.europa.eu/legal-content/EN/ALL/?uri=CELEX:32002D0657.

Zhang Y S，Lin Z H，Hu Y L，Li G K. J. Instrum. Anal.（张艳树，林振华，胡玉玲，李攻科. 分析测试学报），2016，35（2）：127-136.

Yin L H，Xiao X H，Huo B Y，Li G K. J. Instrum. Anal.（尹林华，肖小华，霍冰洋，李攻科. 分析测试学报），2023，42（5）：647-656.

Luo H T，Huang X L，Wu H Q，Zhang Q Y，Zhu Z X，Huang F，Lin X S，Ma Y F，Deng X. J. Instrum. Anal.（罗辉泰，黄晓兰，吴惠勤，张秋炎，朱志鑫，黄芳，林晓珊，马叶芬，邓欣. 分析测试学报），2019，38（5）：532-538.

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