Rapid Screening for 34 Benzodiazepines in Dried Blood Spots Using Direct Analysis in Real Time-Tandem Mass Spectrometry

LIU Fu-bang; ZHANG Ying; WANG Ji-fen; ZHOU Pei-long; HOU Xiao-long

doi:10.12452/j.fxcsxb.23082902

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Rapid Screening for 34 Benzodiazepines in Dried Blood Spots Using Direct Analysis in Real Time-Tandem Mass Spectrometry

Experimental Techniques and Methods | 更新时间：2024-02-21

- Rapid Screening for 34 Benzodiazepines in Dried Blood Spots Using Direct Analysis in Real Time-Tandem Mass Spectrometry
- Journal of Instrumental Analysis Vol. 43, Issue 2, Pages: 309-314(2024)
- 作者机构：
  
  1.中国人民公安大学侦查学院，北京 100038
  2.北京市公安司法鉴定中心，法庭毒物分析公安部重点实验室，北京 100192
  3.中国医学科学院北京协和医学院药物研究所，北京 100050
- 作者简介：
- 基金信息：
- DOI：10.12452/j.fxcsxb.23082902
  CLC： O657.6;R917
- Published：15 February 2024，
  
  Received：29 August 2023，
  
  Revised：20 October 2023，
- 稿件说明：
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刘富邦,张瑛,王继芬等.干血斑中34种苯二氮卓类物质的实时直接分析-串联质谱快速筛查研究[J].分析测试学报,2024,43(02):309-314.

LIU Fu-bang,ZHANG Ying,WANG Ji-fen,et al.Rapid Screening for 34 Benzodiazepines in Dried Blood Spots Using Direct Analysis in Real Time-Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2024,43(02):309-314.
刘富邦,张瑛,王继芬等.干血斑中34种苯二氮卓类物质的实时直接分析-串联质谱快速筛查研究[J].分析测试学报,2024,43(02):309-314. DOI： 10.12452/j.fxcsxb.23082902.

LIU Fu-bang,ZHANG Ying,WANG Ji-fen,et al.Rapid Screening for 34 Benzodiazepines in Dried Blood Spots Using Direct Analysis in Real Time-Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2024,43(02):309-314. DOI： 10.12452/j.fxcsxb.23082902.

摘要

该文通过优化仪器条件和前处理方法建立了实时直接分析-串联质谱快速筛查干血斑（DBS）中34种苯二氮卓类物质（BZDs）的方法。首先将50 µL血液沉积在采血卡上形成DBS，DBS经溶剂充分提取后取上层溶液，使用DART 12Dip-It

模块进样5 µL，在多反应监测模式下检测。结果表明：选用400 ℃作为载气加热器温度，以乙酸乙酯-水（体积比3∶1）混合溶剂提取DBS样品，所得响应强度较高且建立的筛查方法选择性良好，无延迟效应影响，除阿地唑仑、奥沙西泮和α-羟基三唑仑外，其他物质的检出限均在5~50 ng/mL。所建方法可在90.3%的阳性检材中成功筛选出BZDs物质，方法快速、简便、有效，适用于服药自杀等类案件中DBS检材的快速筛查检验。

Abstract

In this paper，a rapid and effective screening method for 34 benzodiazepines（BZDs） in dried blood spots（DBS） using direct analysis in real time-tandem mass spectrometry（DART-MS/MS） was established by optimizing the instrumental conditions and pre-treatment methods. 50 µL of blood was deposited on the blood collection card to form DBS，the upper solution was taken after sufficient solvent extraction and 5 µL was injected using the DART 12Dip-It

module and assayed in multiple reaction monitoring（scheduled MRM） mode. The results showed that：400 ℃ was chosen as the carrier gas heater temperature，and the DBS samples were extracted using a solvent mixture of ethyl acetate-water（3∶1，by volume），and the upper layer of the solution was injected，which showed a high response intensity and good selectivity without the influence of delayed effect. The limits of detection were in the range of 5-50 ng/mL for all substances except for adinazolam，oxazepam and α-hydroxytriazolam. BZDs were successfully screened in 90.3% of the positive case samples. The method is fast，simple，effective and suitable for the rapid screening test of DBS samples in cases such as suicide by drug taking.

关键词

实时直接分析干血斑苯二氮卓类物质快速筛查

Keywords

direct analysis in real timedried blood spotbenzodiazepinesrapid screening

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