Currently

the identification of phosphopeptides mainly depends on mass spectrometry

but the low abundance of phosphopeptide and the interference of non phosphopeptide affect the analysis and identification of mass spectrometry.Therefore

the enrichment of phosphopeptide before mass spectrometry is a prerequisite for further study of phosphoproteomics.This review summarizes the mechanisms and advantages or disadvantages of those traditional and new developed methods for phosphopeptides enrichment

including immobilized metal ion affinity chromatography(IMAC)

metal oxide affinity chromatography(MOAC)

strong cation/anion exchange chromatography(SCX/SAX)

hydrophilic interaction chromatography(HILIC)

ectrostatic repulsion-hydrophilic interaction chromatography(ERLIC)

chemical derivatization

matrix assisted laser desorption/ionization(MALDI) plate specific enrichment method

and coupled methods.