A liquid chromatography-tandem mass spectrometric（LC-MS/MS） method was developed for the quantitative determination of aflatoxin B1（AFB1）

aflatoxin M1（AFM1）

T-2 toxin（T-2）

HT-2 toxin（HT-2）

deoxynivalenol（DON）

ochratoxin A （OTA） and zearalenone（ZEN） in shrimp feed.Samples were extracted with 15 mL acetonitrile-water（4∶1

by volume）

and degreased with 10 mL acetonitrile saturated hexane.The extract was cleaned up with a MycoSpinTM400 multitoxin LC-MS/MS clean-up column.The target compounds were separated on an Hypersil Gold（150 mm×2.1 mm

5 μm）chromatographic column by gradient elution with methanol-water containing 0.03%ammonia as mobile phase

and analyzed simultaneously by electron spray ionization mass spectrometry in positive or negative ion mode

and quantified by the matrix-matched external standard method.Under the optimized conditions

there were good linearities for the analytes in corresponding concentration range with their correlation coefficients（r2） more than 0.991.The limits of detection（LOD）ranged from 1.83 μg/kg to 12.63 μg/kg.Average recoveries for the target mycotoxins at high

medium and low spiked levels ranged from 87.5%to 116%with relative standard deviations of 2.4%-18%.The developed method could provide a reference for the simultaneous determination of mycotoxins in aquatic feeds.