1.烟台大学 生命科学学院,山东 烟台 264000
2.山东省农业科学研究院农产品加工与营养研究所,山东 济南 250000
3.青岛海关技术研究中心,山东 青岛 266100
4.国家食品安全风险评估中心,北京 100022
张鸿伟,博士,研究员,研究方向:食品质量安全检测,E-mail:light04@126.com
肖晶,博士,研究员,研究方向:食品安全标准化,E-mail:xiaojing@cfsa.net.cn
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刘楠,许利丽,张晓梅等.基于高分辨质谱的食品致敏蛋白检测研究进展[J].分析测试学报,2023,42(12):1666-1672.
LIU Nan,XU Li-li,ZHANG Xiao-mei,et al.Research Progress on Detection of Food Allergic Protein Based on High-resolution Mass Spectrometry[J].Journal of Instrumental Analysis,2023,42(12):1666-1672.
刘楠,许利丽,张晓梅等.基于高分辨质谱的食品致敏蛋白检测研究进展[J].分析测试学报,2023,42(12):1666-1672. DOI: 10.19969/j.fxcsxb.23053003.
LIU Nan,XU Li-li,ZHANG Xiao-mei,et al.Research Progress on Detection of Food Allergic Protein Based on High-resolution Mass Spectrometry[J].Journal of Instrumental Analysis,2023,42(12):1666-1672. DOI: 10.19969/j.fxcsxb.23053003.
食品致敏原事关食品安全,其检测技术的进步对于食物过敏风险评估具有重要现实价值。与传统的酶联免疫吸附(ELISA)等免疫学检测方法相比,高分辨质谱技术(HRMS)凭借高通量等特点,近些年在食品致敏蛋白检测研究中的应用日渐广泛。该文主要综述了基于HRMS技术的致敏蛋白检测研究进展,重点介绍了致敏蛋白的提取纯化、酶解以及基于HRMS的分析策略和数据采集模式,同时分析了HRMS在致敏蛋白检测方面存在的挑战,以期为促进致敏蛋白检测的现代化发展提供参考。
Food allergen is tightly related to food safety,and technological progresses on the issue are realistically valuable to food allergy risk assessment. Compared with conventional immunological technique such as enzyme-linked immunosorbent assay(ELISA),high-resolution mass spectrometry(HRMS) is burgeoning in recent years for food allergic protein with its high-throughput characterization. In this paper,the research progress of HRMS technology for allergic protein detection is mainly reviewed,and extraction,purification and enzymatic hydrolysis of allergic protein,as well as HRMS-based analysis strategy and data acquisition is emphatically highlighted. Additionally,the challenges in the detection of allergic protein were also analyzed.
高分辨质谱致敏蛋白检测
high-resolution mass spectrometryfood allergic proteindetection
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