As the variety of new psychoactive substances（NPS） continues to increase and their abuse becomes more and more widespread，their rising popularity poses a serious challenge to national anti-drug agencies．Herein，a direct analysis of real time－tandem mass spectrometry（DART－MS/MS） was developed for the detection of 12 NPS of benzodiazepines（DBZDs） in urine．The MS conditions，including injection volume，carrier gas heater temperature and pretreatment method were optimized．Methodological validation was performed．The results showed that the best pretreatment effect was obtained by liquid-liquid extraction using ethyl acetate．Then，5 µL supernatant was pipetted into the bottom of a glass rod，and a DART 12Dip－It™ sampler was used for sampling．The best response was obtained by analysis in positive ionization mode under multiple reaction monitoring（MRM） mode at a carrier gas heater temperature of 300 ℃．The quantitation analysis of DBZDs was performed by internal standard method with diazepam-D,5, as the internal standard．The linear relationships for 12 DBZDs were good in the range of 0.05－5 μg/mL，with correlation coefficients（,r,2,） great than 0.99．The limits of detection and the limits of quantitation were in the range of 0.5－20 ng/mL and 1－50 ng/mL，respectively．The average matrix effects were in the range of -11.1%－12.6%．The average recoveries ranged from 94.7% to 115%，with intra-day relative standard deviations（intra-RSDs） and inter-RSDs for high and medium concentrations both not more than 15%，as well as the intra-RSDs and inter-RSDs for concentration of quantitative limit not more than 17%．The method is rapid，simple and reproducible，and could meet the requirements for rapid detection of 12 NPS BZDs in urine in the public security field.