1.遵义师范学院 生物与农业科技学院,贵州 遵义 563006
2.遵义师范学院 资源与环境学院, 贵州 遵义 563006
3.湖南科技大学 化学化工学院,湖南 湘潭 411201
周建红,博士研究生,研究方向:分析化学,E - mail:jianhongzhou@163.com
扫 描 看 全 文
周建红,令玉林,曾伯平等.基于溴化银-银-碳纳米管复合物与核酸适配体的阴极光电化学传感器检测赭曲霉素A[J].分析测试学报,2022,41(08):1253-1258.
ZHOU Jian-hong,LING Yu-lin,ZENG Bo-ping,et al.Determination of Ochratoxin A Using a Cathodic Photoelectrochemical Sensor Based on AgBr-Ag-CNTs Composite and Aptamer[J].Journal of Instrumental Analysis,2022,41(08):1253-1258.
周建红,令玉林,曾伯平等.基于溴化银-银-碳纳米管复合物与核酸适配体的阴极光电化学传感器检测赭曲霉素A[J].分析测试学报,2022,41(08):1253-1258. DOI: 10.19969/j.fxcsxb.22031601.
ZHOU Jian-hong,LING Yu-lin,ZENG Bo-ping,et al.Determination of Ochratoxin A Using a Cathodic Photoelectrochemical Sensor Based on AgBr-Ag-CNTs Composite and Aptamer[J].Journal of Instrumental Analysis,2022,41(08):1253-1258. DOI: 10.19969/j.fxcsxb.22031601.
该研究利用水热法制备了一种新的溴化银-银-碳纳米管(AgBr-Ag-CNTs)复合材料,并通过扫描电子显微镜、X-射线衍射和X-射线光电子能谱对其进行表征。以AgBr-Ag-CNTs作为光电阴极,相比于单一的AgBr和二元的AgBr-Ag复合材料,其具有更高的光电化学活性。采用K,3,[Fe(CN),6,]作为光电子受体能进一步提高光电流信号。基于AgBr-Ag-CNTs为光电阴极传感基底,构建了一种灵敏检测赭曲霉素A (OTA)的核酸适配体传感器。对目标物的线性浓度范围为4.0 pg/mL ~ 2.5 ng/mL,相关系数(,r,2,)为0.994,检出限为1.5 pg/mL。该传感器制备简单、成本低、灵敏度高、抗干扰强,对样品检测的回收率为92.0% ~ 108%,可用于实际样品中OTA的分析。
Photocathode-based sensors possess the advantages of high selectivity and anti-interference in real sample detection,though photocathode produces low photocurrent signal and gives rise to poor sensitivity.Herein,a novel silver bromide-silver-carbon nanotubes(AgBr-Ag-CNTs) composite was synthesized by solvothermal method,and was characterized by scanning electronic microscopy,X-ray diffraction and X-ray photoelectron spectroscopy.Because reduced Ag has revealed localized surface plasmon resonance effect(LSPR),it can amplify light absorption efficiency.Furthermore,CNTs could optimize the electric field near metallic Ag,strengthen light absorption and improve the separation efficiency of photo-generated carriers.Thus AgBr-Ag-CNTs displayed an better cathodic photoelectrochemical activity,compared with AgBr or AgBr-Ag.With K,3,[Fe(CN),6,] as photoelectron acceptor,the photocurrent response could be further increased.To develop the practicality of AgBr-Ag-CNTs,an ochratoxin A(OTA) aptasensor was constructed by using AgBr-Ag-CNTs as photocathodic sensing interface,an aptamer as target recognition site,and K,3,[Fe(CN),6,] as photoelectron acceptor.Since the aptamer/OTA complex has a rigid structure with weak conductivity,it could effectively block the absorption of light with the sensing interface,inhibit the transfer of photoelectrons toward electron receptors,and reduce the photocurrent signal.Based on the changed photocurrent as signal,the sensor showed a wide linear response from 4.0 pg/mL to 2.5 ng/mL(,r,2,= 0.994) and a low detection limit of 1.5 pg/mL.It was used for detection of OTA in real samples,with recoveries of 92.0%-108%.This work provided a novel photoelectrochemical method for OTA detection,with high sensitivity,specificity and good anti-interference.
溴化银-银-碳纳米管光电阴极传感器核酸适配体赭曲霉素A
AgBr-Ag-CNTsphotocathode sensoraptamerochratoxin A
Kumar P,Mahato D K,Sharma B,Borah R,Haque S,Mahmud M M C,Shah A K,Rawal D,Bora H,Bui S.Toxicon,2020,187:151-162.
Chrouda A,Sbartai A,Baraket A,Renaud L,Maaref A,Jaffreaic-Renault N.Anal. Biochem.,2015,488:36-42.
Regulation E C.Off. J. Eur. Union,2006,L364:5-18.
Qian M Y,Hu W X,Wang L H,Wang Y,Dong Y F.Toxins,2020,12(6):376-382.
Campone L,Piccinelli A L,Celano R,Pagano I,Russo M,Rastrelli L.Food Chem.,2018,244:128-135.
Armutcu C, Uzun L, Denizli A. J. Chromatogr. A,2018,1569:139-148.
Robinson A L,Lee H J,Ryu D.Food Chem.,2017,214:47-52.
Zhang T,Xing B,Han Q Z,Lei Y F,Wu D,Ren X,Wei Q.Anal. Chim. Acta,2018,1032:114-121.
Qing Y,Li X,Chen S,Zhou X P,Lou M,Xu X,Li C R,Qiu J F.Microchim. Acta,2017,184:863-870.
Wei M,Zhang W Y.Sens. Actuators B,2018,276:1-7.
Suea-Ngam A,Howes P D,Stanley C E,DeMello A J.ACS Sens.,2019,4:1560-1568.
Shu J,Tang D P.Chem. Asian J.,2017,12:2780-2789.
Shu J,Tang D P.Anal. Chem.,2020,92:363-377.
Xu Y T,Yu S Y,Zhu Y C,Fan G C,Han D M,Qu P,Zhao W W.TrAC,Trends Anal. Chem.,2019,114:81-88.
Deng K Q,Xiao J,Liu Z,Li C X,Wang J L,Yi Q F,Huang H W,Zhou H.Biosens. Bioelectron.,2021,181:113152.
Xiao J,Deng K Q,Liu Z,Li C X,Wang J L,Yi Q F,Huang H W,Zhou H.Sens. Actuators B,2021,348:130691.
Zhang J,Xu X J,Qiang Y.Sens. Actuators B,2020,312:127964.
Deng K Q,Liu X Y,Li C X,Hou Z H,Huang H W.Sens. Actuators B,2017,253:1-9.
Lin H L,Cao J,Luo B D,Xu B Y,Chen S F.Catal. Commun.,2012,21:91-95.
Lin S L,Liu L,Hu J S,Liang Y H,Cui W Q.Appl. Surf. Sci.,2015,324:20-29.
0
浏览量
5
下载量
0
CSCD
关联资源
相关文章
相关作者
相关机构