A liquid chromatography-mass spectrometry with microdialysis was used to screen potential α glucosidase inhibitors in the extracts of Scutellaria baicalensis and identify the structures of those bioactive components screened.The chromatographic separation was carried out on a Dikma C18 column(150 mm×46 mm,5 μm) by gradient elution with 05% acetic acid solution-acetonitrile as mobile phases at a flow rate of 05 mL/min and a column temperature of 30 ℃.Mass spectrometry analysis and data acquisition were conducted in the positive ion mode.In this work,ten potential α glucosidase inhibitors in Scutellaria baicalensis were found and identified simultaneously.The binding degrees of 10 compounds with α glucosidase ranged from 3618% to 5271%.With the advantages of rapidness,high throughput and effectiveness,this method could screen α glucosidase inhibitors in Scutellaria baicalensis and provide valuable information for screening α glucosidase inhibitors from other traditional Chinese medicines.