Metabolomics is a powerful tool for small molecule metabolites analysis,and it is playing a more and more important role in the precision medicine and translational medicine.Mass spectrometry(MS) coupled with different chromatographic modes have become the pillar technology platforms for metabolomics due to their high sensitivity,good selectivity,wide dynamic range and rich information.Innovations and developments of these analytical methods are the important prerequisites for wide applications of metabolomics in various fields.Advances of the analytical methods focused on liquid chromatography-mass spectrometry based metabolomics technique in the past five years are summarized in this review,and the existing problems and future perspectives of this technique are also discussed.81 references are cited.