A urinary metabonomics method based on ultra high performance liquid chromatography coupled with quadrupole time of flight mass spectrometry(UHPLC-Q-TOF MS) was established for the investigation on mechanism of Radix Scutellariae-Gardenia jasminoides couples treating diabetic nephropathy.A diabetic model was established by streptozotocin intraperitoneal injection and feeding with high fat and high sucrose chow.After intragastric administration for 12 weeks,UHPLC-Q-TOF MS was used to analyze the metabolic profiles of the urinary samples from the control group,the model group and the Radix Scutellariae-Gardenia jasminoides Couples treatment group,which were then compared with the results from the Radix Scutellariae treatment group and the Gardenia jasminoides treatment group.The data were analyzed by Orthogonal partial least-squares discriminant analysis(OPLS-DA).According to the results of OPLS-DA，the compounds providing greater contribution to separation of each group were searched from the HMDB databases.Finally,31 potential biomarkers were identified,and 16 of them were related to the RadixScutellariae-Gardenia jasminoides Couples.Results revealed that Radix Scutellariae-Gardenia jasminoides couples played a role in diabetic nephropathy treatment through mainly affecting the pathways of tryptophan metabolism,phenylalanine metabolism,tyrosine metabolism,energy metabolism and biosynthesis of bile acids,in which biosynthesis of bile acids,urotoxin metabolism and energy metabolism are the important pathways to treat diabetic nephropathy.Compared with the single drug of Radix Scutellariae or Gardenia jasminoides,Radix Scutellariae-Gardenia jasminoides couples had the special treatment effect.