Lipids play important roles in a variety of critical biological processes.Therefore,significant research efforts have been devoted to the structural characterization and functional analysis of lipids.Although mass spectrometry(MS) has become one of the most common techniques for rapid and sensitive analysis of lipids,the structural characterization on lipids remains to be an analytical challenge.Aiming to solve this problem,an effective and widely adopted method was developed for localizing C‖C(s) in unsaturated lipids and quantifying lipid C‖C location isomers.Besides,this method could be readily coupled with shotgun lipidomics,liquid chromatography-mass spectrometry(LC-MS) and MS imaging,to achieve detailed lipid structural analysis on various types of biological samples.