The issue of pesticide residues in Chinese medicinal materials has always been a focus of attention.As an important rapid analysis technique with strong specificity and high sensitivity,immunoassay plays an important role in the rapid screening of pesticide residues in Chinese herbal medicine.Combined with the relevant research progress on immunoassay in recent years,the application,technical advantages and limitations of different immunoassay techniques in pesticide residue analysis in Chinese herbal medicine are analyzed and summarized in this review,and the prospect for the development of immunoassay technique is also forecasted.This review has an important instructive to the application of immunoassay in pesticide residue analysis and the safety evaluation of traditional Chinese medicines.