A rapid method was established for the nondestructive determination of multi marker components in Aconiti Lateralis Radix Praeparata by near infrared spectroscopy(NIRS) combined with partial least squares(PLS) and least square-support vetor machine(LS-SVM).A total of 38 batches of samples were selected for the determination of six alkaloids,ie.benzoylmesaconine,benzoylaconine,benzoylhypaconine,meaconitine,hypaconitine and aconitin in Aconiti Lateralis Radix Praeparata by high performance liquid chromatography(HPLC),and their NIR spectra were further collected.Two determination calibration models between the six components and NIRS were established by PLS and LS-SVM methods,respectively.The relative prediction deviations(RPDs) for the six components the total of monoester type alkaloids and the total of diester type alkaloid were 3.3,3.2,4.1,7.7,8.8,7.6,4.0 and 8.6,while the rpre for the 8 types were 0.948 6,0.947 5,0.966 8,0.990 9,0.994 6,0.996 9,0.966 9 and 0.992 7,respectively.Results showed that the LS-SVM model was better than PLS model,indicating that a good nonlinear relation existed between the predicted values of NIRS model and the measured values of HPLC.The established NIRS combined with LS-SVM could be applied to the rapid determination of six alkaloids in Aconiti Lateralis Radix Praeparata,and even to the prediction of the total of monoester type alkaloids and the total of diester type alkaloid,which is beneficial for the quality control of the Aconiti Lateralis Radix Praeparata.