Traditional Chinese medicines(TCMs) play a significant role due to their less adverse reactions and unique medical efficiency,which have attracted more and more attention both at home and abroad.However,TCMs are prone to mildew and generate aflatoxins in production,collection,processing,transportation and storage.Aflatoxins with strong toxicity could not only affect the quality and efficacy of traditional Chinese medicines,but also cause great damage to the liver and other organs of human.A rapid dynamic surface enhanced Raman spectroscopic(D-SERS) method was established for the detection of aflatoxin G1(AFG1) in coix seed,a kind of traditional Chinese medicine.The aflatoxin G1 on the surface of coix seed was extracted by wiping method,while not all of the ingredients in coix seed could be extracted.Therefore,there was less interference of coix seed in SERS spectrum.Aflatoxin G1 was analyzed by D-SERS with gold nanoparticles as the enhanced substrate,which are characterized by their good stability.The detection conditions were optimized.The calibration curves were linear in the range of 8-320 μg/kg,with a linear formula as y=0.029 3x+1.730 7,and a correlation coefficient(r2) of 0.988 4.The limit of detection(LOD) was 5.5 μg/kg,and the relative standard deviation(RSD) was 11%.Results showed that the established method was simple,rapid and cost saving,and it could meet the requirements for rapid detection of aflatoxin G1 in coix seed with good linearity,sensitivity and reproducibility.Therefore,this method has a good application potential in real time and rapid analysis of aflatoxin G1 in Chinese herbal medicines.