Ru（bpy）2+3 has an excellent electrochemiluminescence（ECL） properties.However,the good solubility makes it meet a huge problem with the immobilization.In this experiment,an ECL biosensor for detection of glucose was fabricated by modifying Pt NPs-Ru complexes composed of Pt nanoparticles and Ru（bpy）2+3 onto the electrode surface for immobilization of glucose oxidase（GOx）.In fact,the ECL signal of Ru（bpy）2+3-triethylamine system could be efficiently quenched by H2O2.With increase of glucose concentration,amount of H2O2 generated in situ by glucose under the catalysis of GOx increased,and the ECL signal gradually decreased,thus achieving the detection of glucose.A good linear relationship between ECL intensity and the logarithm of glucose concentration in the range of 1.0×10-8-5.0×10-5 mol/L was obtained,with a detection limit of 5.2×10-9 mol/L.The biosensor showed good stability and high selectivity.The Pt NPs-Ru complexes provide a good platform for construction of ECL sensor and a new method for detection of glucose.