An ultra high performance liquid chromatography-tandem mass spectrometric(UPLC-MS/MS） method was developed for the simultaneous determination of 23 bisphenol analogs and their halogenated derivatives(BPs） in urine and serum based on derivatization of pyridine-3-sulfonyl chloride.Urine samples were extracted by liquid-liquid extraction with ethyl acetate,while serum samples were extracted with acetonitrile and followed by PRiME HLB cartridge purification.After derivatization with pyridine-3-sulfonyl chloride,the extracts were separated on an ACQUITY UPLC BEH C18(2.1 mm×100 mm，1.7 μm） column with acetonitrile and water containing 0.1% formic acid as mobile phases,and determined by UPLC-MS/MS with electrospray ionization(ESI） in positive ion mode.There were good linear relationships for 23 BPs in both urine and serum samples in the range of 0.005-100 μg/L,with their limits of detection between 0.002 μg/L and 0.030 μg/L ，limits of quantitation between 0.005 μg/L and 0.100 μg/L.Average recoveries ranged from 76.6% to 122%,with relative standard deviations(RSD） of 0.60%-14%.The method was applied in the analysis of BPs in 20 urine samples and 20 serum samples.The method could meet the requirements for simultaneous determination of 23 BPs in urine and serum samples with low sample consumption and high sensitivity.