1. 合肥工业大学食品与生物工程学院
2. ,内蒙古,呼和浩特,010020
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俞婷, 周宝梅, 徐敏, 等. 食源性致病菌的表面增强拉曼光谱检测技术研究进展[J]. 分析测试学报, 2021,40(4):519-527.
Advances in Surface Enhanced Raman Spectroscopic Techniques for Detection of Foodborne Pathogens[J]. 2021,40(4):519-527.
食源性致病菌引起的疾病的快速管控与预防是当前各国面临的食品安全监管难题之一,受到社会各界的广泛关注。目前常用的食源性致病菌检测方法存在步骤复杂、耗时、灵敏度低或选择性差等局限,发展快速、可靠的食源性致病菌检测方法仍是食品安全和公众健康的热点研究领域。表面增强拉曼光谱(SERS)作为一种新型的光谱快检技术,具有灵敏度高、选择性好、快速、无损检测等优点,在食源性致病菌检测方面表现出广阔的应用前景。该文简要介绍了SERS技术的背景、增强机制并总结了用于致病菌检测的活性纳米结构,全面综述了近5年来利用直接或间接SERS法以及SERS与其他技术结合检测食源性致病菌的研究进展,同时讨论了这些方法的优缺点。最后,提出当前SERS技术在实际应用中面临的挑战,并展望了SERS在食源性致病菌检测领域未来可能的发展趋势。
The rapid control and prevention of diseases caused by foodborne pathogens is one of the existing food safety supervision issues in both developed and developing countries,which has widely attracted attention from all sections of the community.Up to now,there are some limitations on commonly used methods for detection of foodborne pathogens such as complex steps,time consuming,low sensitivity,poor selectivity,etc.Therefore,development of rapid and reliable methods for detection of foodborne pathogens is still a hot research area for the sake of food safety and public health.As a novel spectroscopic detection technology,surface enhanced Raman spectroscopy(SERS) has shown a broad prospect in the detection of foodborne pathogens with the advantages of high sensitivity,selectivity,rapidness and non destructive detection.In this article,the background and enhanced mechanism of SERS are briefly introduced first,then the SERS active nanostructures used for foodborne pathogen detection are summarized.Furthermore,a comprehensive overview on research progress of direct and indirect SERS methods applied to detection of foodborne pathogens in the past five years is provided,as well as the combination of SERS and other techniques.Meanwhile,the advantages and disadvantages of different SERS substrates and methods are discussed.Finally,the current challenges of SERS technology applied in practical applications are listed in this article,and the possible development trends of SERS in the field of foodborne pathogens detection in the future are put forward.
表面增强拉曼光谱食源性致病菌无标签检测基于标签检测
surface enhanced Raman spectroscopyfoodborne pathogenslabel free methodlabel method
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