Advances on Application of Rolling Circle Amplification-mediated Nucleic Acid Signal Amplification Strategy in Biosensors[J]. 2020,39(12):1556-1560.DOI:
Rolling circle amplification(RCA) is an isothermal nucleic acid amplification technique.Due to its high efficiency and high fidelity,this technique has been widely used in biosensors.This review introduces the principle of RCA technique,the common cyclization types of RCA template and the common types of RCA,as well as the fluorescent,colorimetric and electrochemical biosensors based on RCA.The aim of this review is to provide references for the further development and application of the RCA technique in biosensors.
关键词
等温核酸扩增滚环扩增生物传感器
Keywords
isothermal nucleic acid amplificationrolling circle amplificationbiosensor