An ultra performance liquid chromatography-tandem mass spectrometric(UPLC-MS/MS) method was established for the determination of 73 glucocorticoids in cosmetics．Samples were dispersed with saturated sodium chloride and ultrasonically extracted with acetonitrile,then purified with a PRiME HLB SPE column(60 mg/3 mL) and separated on a Waters Cortecs C18 column(150 mm×2.1 mm，2.7 μm) by gradient elution using 0.1% acetic acid solution and acetonitrile as mobile phases.The analysis was performed with electrospray ion source in positive ion mode under multi-reaction monitoring(MRM) mode,and the results were quantified by external standard method.Results showed that there were good linear relationships for 73 glucocorticoids in cosmetics in their respective concentration ranges with correlation coefficients(r) higher than 0.99.The limits of quantitation(LOQ) were in the range of 0.1-0.3 μg/g．The average recoveries for 73 glucocorticoids at three spiked levels of 1LOQ,2LOQ and 5LOQ ranged from 73.3% to 119%,with relative standard deviations(RSD) of 0.40%-18%.The method was rapid and accurate,and could be used as a screening approach for 73 glucocorticoids in cosmetics.