An ultra-performance liquid chromatography-tandem mass spectrometric(UPLC-MS/MS) method was established for the simultaneous determination of 35 sex hormones in cosmetics.The sample was ultrasonically extracted with 50% acetonitrile solution,and separated on a CORTECS C18(2.1 mm×150 mm，2.7 μm) column by gradient elution using acetonitrile-water as mobile phases.The analysis was performed under multiple reaction monitoring(MRM) mode. Results showed that there were good linear relationships for 35 sex hormones in the respective concentration range with their correlation coefficients(r) all above 0.99．The limits of detection(LOD) and limits of quantitation(LOQ) for 35 sex hormones were in the ranges of 0.028-0.117 μg/g and 0.093-0.352 μg/g,respectively.Average recoveries of toner,emulsion and cream samples at three levels of 0.25,0.50,1.00 μg/g were in the range of 84.0%-117%，with relative standard deviations(RSD,n=6) of 1.5%-14%.With the advantages of simplicity,sensitivity and accuracy,this method is suitable for the analysis of sex hormones in cosmetics.