A nano platinum/reduced graphene oxide modified glassy carbon electrode(Pt/E-rGO/GCE) was prepared by one step electrodeposition using a mixture of graphene oxide and chloroplatinic acid as the precursor.The electrochemical behavior of 1 hydroxypyrene on the modified electrode was studied,and a sensitive accumulation and detection of 1-hydroxypyrene was performed by differential pulse voltammetry.Results showed that the response current for 1-hydroxypyrene reached the maximum when the solution was set at pH 2.0 and the number of electrodeposition cycles was 5.The concentration of 1 hydroxypyrene showed a good relationship with the reduction peak current in the range of 50-700 nmol/L with a correlation coefficient(r) of 0.976 and a limit of detection(S/N=3) of 12.1 nmol/L.The Pt/E-rGO/GCE exhibited good stability,reproducibility and anti-interference ability,and was applied in the determination of 1-hydroxypyrene in human urine with recoveries of 99.7%-108%and relative standard deviations of 3.2%~4.2%.The good performance of the Pt/E-rGO/GCE provides a certain practical value for the construction of an electrochemical sensor.