A rapid analytical method for the simultaneous determination of 7 lincosamides and macrolides residues in honeys was developed by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The samples were dissolved with phosphate buffer(pH 8.0),then extracted with 1% ammonia acetonitrile and salted out with anhydrous sodium sulfate.The extract was cleaned up with 80 mg ZnO and 20 mg PSA.The target compounds were separated on a Waters XBridge C18(150 mm×2.1 mm，5 μm) column with 0.1% formic acid solution and acetonitrile as mobile phases,then determined with electrospray ionization source in positive ion mode under multiple reaction monitoring(MRM) acquisition mode,and quantified by external standard method using matrix matched standard solution.Results showed that the 7 target compounds showed good linearity in their respective mass concentration ranges with correlation coefficients(r) not less than 0.999 3.The limits of detection of the method were in the range of 0.21-0.35 μg/kg,while the limits of quantitation were 0.69-1.16 μg/kg.At three spiked levels of 5.0,10.0 and 20.0 μg/kg,the average recoveries for 7 target compounds were in the range of 75.5%-99.0% with relative standard deviations of 0.78%-5.9%.The method could be applied to the rapid determination of 7 lincosamides and macrolides residues in honeys with the advantages of simplicity,rapidness,high sensitivity and good reproducibility.