A method was established for simultaneous detection of 97 pesticides in human blood by gas chromatography-tandem mass spectrometry(GC-MS/MS) with Simple-QuEChERS Nano.05 mL of blood sample was added to a 15 mL conical bottom polypropylene centrifuge tube and mixed with 15 mL water,then extracted with 20 mL ethyl acetate.The tube was oscillated for 10 min at 20 r/min,and then centrifuged at 8 000 r/min for 10 min.After that,the supernatant of extracts was purified by filtration through a Simple-QuEChERS Nano column and 022 μm organic membrane filter,then detected in multiple reaction monitoring(MRM) mode.There were good linear relationships for all the analytes except fenitrothion,with their correlation coefficients(r2) no less than 0987 3.The limits of detection(LODs) and the limits of quantification(LOQs) for 96 pesticides except allethrin(LOD and LOQ was 1103,3676 ng/mL) ranged from 006 ng/mL to 427 ng/mL and 018 ng/mL to 1424 ng/mL,respectively.At three spiked levels of 100,200,400 ng/mL,the recoveries for 97 kinds of pesticides ranged from 322% to 120%.The intra day precision and inter day precision of the method evaluated at a concentration of 100 ng/mL ranged from 19% to 11% and 36% to 13%,respectively.The developed method,optimized by the traditional QuEChERS method,was not only environmentally friendly,but also improved the efficiency of sample pre treatment and exhibited an enhanced performance in purifying blood sample,which could be used for the qualitative and quantitative analysis of multi pesticides in blood samples of forensic cases.