A gas chromatographic method coupled with electron capture detection（GC/ECD） was developed for the determination of flumetralin deliver amount in total particulate matter(TPM) of mainstream cigarette smoke during cigarette smoking. Under the standard conditions，cigarette were smoked to collect the TPM of mainstream cigarette smoke with cambridge filter,and then extracted with acetonitrile. The extracts were cleaned up with the SupelcleanTM ENVITM Carb SPE tubes or the mixed chromatography column，and determined by GC/ECD with the external standard method. The calibration curve was linear for flumetralin in the range of 002-20 mg/L with a limit of detection（LOD） of 914×10-6 g/L. When a spiked level of flumetralin by 30 μg/bag was added,the mean recoveries of flumetralin deliver amount in TPM of mainstream cigarette smoke during smoking ranged from 88% to 93% with relative standard deviations （RSDs） of 15%-28%. The average recoveries of flumetralin from spiked blank sample were in the range of 94%-100% with RSDs of 14%-24%. Compared with the traditional methods，the proposed method was simple，rapid，accurate and environment friendly.Therefore,it was suitable for the determination of flumetralin deliver amount in TPM of mainstream cigarette smoke during smoking.