An analytical method based on microemulsion liquid chromatography（MELC） was developed for the simultaneous determination of synephrine，evodiamine and rutecarpine in Fructus Evodiae.The samples were prepared by methanol sonication.The main factors influencing the separation selectivity，including the types and concentrations of surfactant，pH values of mobile phase and the types of organic solvent additive，were investigated.The best composition of the microemulsion system was 3.0% SDS-6.0% n butanol-0.6% n octane -1.0% formic acid -1.2% acetonitrile -88.2% water.Three target compounds were separated with Diamonsil C18 column（250 mm×4.6 mm，5 μm） at a flow rate of 0.7 mL/min.The detection wavelength was set at 260 nm.Under the optimal conditions，the calibration curves of synephrine，evodiamine and rutecarpine were linear in the range of 5.21-26.05，7.04-35.2，1.31-6.55 mg/L，respectively，with their correlation coefficients more than 0.999 7.The average recoveries of three components were between 96% and 101% with RSD（n=6） of 1.0%-1.5%.The limits of detection ranged from 0.048 to 0.095 mg/L.This method was simple，accurate and reliable，and could be applied in the determination of three components in Fructus Evodiae.