A new method of microfluidic chip with contactless conductivity detection was established for the determination of amantadine hydrochloride in amantadine hydrochloride tablets．The electrophoretic parameters，such as the variety and concentration of buffer solution and additive，separation voltage and injection time etc，were investigated．Under the optimal conditions，using 1 mmol/L HAc+2 mmol/L NaAc（pH 4.5）+0.1 mmol/L SDS as buffer solution system，amantadine hydrochloride was separated and detected in 1 min at the separation voltage of 2.00 kV and 10 s injection time．The linear dependence of the concentration of amantadine hydrochloride ranged from 10 mg/L to 120 mg/L （r=0.999 0），with a detection limit（S/N=3） of 0.6 mg/L.The spiked recoveries ranged from 97% to 98% with RSDs of 0.8%-2.2%.The method was simple，rapid and well reproducible，and could be used for the rapid detection and the quality control of the product.