As a new composite material, poly(dimethylsiloxane)-gold nanoparticles (PDMS-AuNPs) composite with dual merits of PDMS and gold nanoparticles has been widely applied in the fields of biosensing,biofunctional materials,environmental protection and microfluidics.In this paper,two main methods for the preparation of PDMS-AuNPs composite in common chemical laboratories and its applications in biosensing were reviewed.The development and application prospect of PDMS-AuNPs composite was put forward.