An analytical method was developed and validated for the simultaneous determination of 3 tetracyclines and 2 quinolones in pork and chicken by ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） by positive ion mode under multiple reaction monitoring （MRM）.The sample were extracted by ultrasonic extraction in Mcllvaine-Na2EDTA buffer （pH 4.0）.The extract was cleaned up on a solid phase extraction（SPE） cartridge packed with multiwalled carbon nanotubes（MWCNTs） and using formic acid- acetonitrile（5∶95，by volume） mixture as eluting solvent.The drugs residues were analyzed by UPLC-MS/MS.The analysis time was only 10 min.Good linearities were obtained in the range of 5-500 μg/L.The limit of detection （LOD） was 15 μg/L for chlortetracycline（CTC） and 5 μg/L for the other four drugs.The recoveries at spiked levels of 50,100,200 μg/L ranged from 76% to 90% for 5 drugs with relative standard deviations（RSDs） less than 9%.With good stability and high sensitivity,the method could be used for the rapid determinaion of these drug residues in pork and chicken sample.