The tests of single factor,orthogonal and verification to separate and purify chlorogenic acid from honeysuckle crude extracts by NKA 2，D1400，polyamide，HP2MGL，ADS 21，D101 and AB 8 seven macroporous resin were carried out in sequence,and the best processing parameters were optimized based on the adsorption and desorption results.The results showed that NKA 2 was the best resin,whose static saturated adsorption capacity was 21217 mg/g,and desorption rate by 90% ethanol reached 814%.The best processing parameters for separating and purifying chlorogenic acid from honeysuckle crude extracts by NKA 2 resin were as follows:sample concentration:4 g/L,sample volume:35 BV,sample flow rate:3 BV/h,elution ethanol concentration:80%,elution volume:3 BV,and elution flow rate:1 BV/h.Under the optimal conditions,the yield rate of chlorogenic acid reached 8579%,and its purity was 4550%.When using sample concentration of 3 g/L,sample volume of 45 BV,sample flow rate of 2 BV/h,elution ethanol concentration of 80%,elution volume of 3 BV and elution flow rate of 3 BV/h,the purity of chlorogenic acid reached 5307%,and its yield was 7333%.Comparing with the current separating and purifying process of chlorogenic acid from honeysuckle crude extracts,the optimized process has better practical and reference value.