The molecularly imprinted monolithic column（MIPMC） has been attracting intensive attention in recent years as it combines the high stereo-selectivity of molecularly imprinted polymers with the advantages of excellent hydrodynamic characteristic，low flow resistance as well as simple preparation of monolithic materials，leading to potential applications in sample pretreatment and chromatographic analysis.In this paper，the methods of the preparation of molecularly imprinted monolithic column in a conventional chromatographic column and capillary column were introduced，and its applications in sample pre-concentration and chromatographic analysis in recent five years were summarized.The development trend of molecularly imprinted monolithic column was also prospected.