Field-amplified sample injection and sweeping-micellar electrokinetic chromatography（FASI-sweeping MEKC） was used in the simultaneous separation and determination of caffeic acid and chlorogenic acid in honeysuckle.Effects of experimental conditions,such as concentration of SDS,injection voltage,injection time ratio of water to sample,injection time,composition and concentration of background electrolyte on separation efficiency were optimized.The optimal conditions were as follows:separation column:an uncoated fused silica capillary column（50 cm×50 μm,effective length 33 cm）,background electrolyte:100 mmol/L sodium dodecyl sulfate（SDS）-20 mmol/L NaH2PO4（pH 2.2）-15% acetonitrile,temperature:25 ℃,detection wavelength:215 nm,sample injection voltage:-10 kV,separation voltage:-20 kV,sample injection time:15 s,water injection time:195 s（H=20.0 cm）.Under the optimum conditions,the separation of the two organic acids was performed for 15 min with RSDs of peak area less than 4%.The calibration curves showed good linearities in the ranges of 29.4-470.4 μg/L for caffeic acid and 48.5-776 μg/L for chlorogenic acid with detection limits（S/N=3）of 1.12 μg/L and 2.18 μg/L,respectively.The spiked recoveries were in the range of 98%-106% and 96%-106%,respectively.