Using the characteristics that subcritical water can quantitatively extract a wide variety of organic solutes from different matrixes,12 pesticides were extracted from radish,cantaloupe,apple and cabbage by subcritical water extraction combined with solid phase adsorption in accelerated solvent extractor after adding C18 padding in the cell.The extraction conditions were optimized.The determination of 12 pesticides was performed by gas chromatography-tandem mass spectrometry(GC-MS/MS).The result indicated that the limits of quantitation were in the range of 0.025-0.050 mg/kg,and the recoveries of 12 pesticides from fruits and vegetables samples at spiked level of 0.05 mg/kg were in the range of 62%-110% with RSDs less than 10%.