Electrochemical behavior and response of uric acid（UA） was investigated at the chemically reduced graphene oxide（RGO）modified glassy carbon（GC） electrode（RGO/GC） by cyclic voltammetry,chronocoulometry and AC impedance method.The results showed that，in pH 60 PBS buffer solution,the electrochemical oxidation of UA at the RGO/GC modified electrode was irreversible and controlled by diffusion.Some kinetic parameters such as the number（n） of the electron transferred,the effective area（A） of the modified electrode and the diffusion coefficient（D） were calculated to be 2,0.182 cm2 and 1.51×10-6 cm2?s-1,respectively.A good linear relationship between peak current and concentration of UA was observed in the range of 5.0×10-6-1.5×10-4 mol/L,which met the equation of Ip（μA） =6.457+367.5c（mmol/L）（r=0.995 7）.The RGO/GCE modified electrode was successfully applied in the determination of UA with a detection limit of 2.7×10-7 mol/L and recoveries of 98%-100% .